The Structure of the Nuclear Pore Complex: Supplemental Video 2

A supplemental video from the 2011 review by André Hoelz, Erik W. Debler, and Günter Blobel, "The Structure of the Nuclear Pore Complex," from the Annual Review of Biochemistry.

Active import and export of cargoes are facilitated by nuclear localization and nuclear export sequences (NLS and NES, respectively) that are recognized by transport factors, collectively termed karyopherins (Kaps). The NLS of import cargoes (blue) is recognized either directly by an import karyopherin-β (Kap-β; salmon) or via an adapter karyopherin (Kap-α; light green). RanGTP (red) binding inside the nucleus leads to dissociation of the import complex. By contrast, the assembly of a NES-cargo Kap-β export complex requires RanGTP binding (represented in blue, yellow, and red, respectively). In the cytosol, this export complex is dissociated by GTP hydrolysis, which is catalyzed by Ran GTPase-activating protein (RanGAP; dark green) or Ran-binding protein 1 (RanBP1). Artwork by Joseph Alexander, Erik W. Debler, and André Hoelz.