High-Speed AFM and Applications to Biomolecular Systems: Supplemental Video 4

A supplemental video from the 2013 review by Toshio Ando, Takayuki Uchihashi, and Noriyuki Kodera, "High-Speed AFM and Applications to Biomolecular Systems," from the Annual Review of Biophysics.

Long tracking of myosin V (M5-HMM) walking along actin filament. This typical movie showing long processive runs in 1 µM ATP was captured at 7 fps. To chase the M5-HMM molecule, the scan area was moved. Scan range, 150 × 75 nm2 with 80 × 40 pixel; the whole imaging area, 560 × 120 nm2; number of steps observed, 14. Foot stomp events at the leading head are marked with light-blue triangles.