Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 1

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: Biphasic neutrophil swarming response to focal tissue damage

Bone marrow-derived neutrophils from C57BL/6 mice were CMFDA-labeled and injected intradermally into the ventral ear skin of a Tyr^c-2J/c-2J mouse 3 h before laser-induced focal tissue damage. This representative video shows the biphasic chemotactic response of neutrophils (pseudo-colored in green) sensing the focal tissue damage (autofluorescence, green) (left) within the fibrous collagenous connective tissue of the ear skin dermis (visualized by second harmonic generation, white) (right).

Original publication: Lämmermann T, Afonso PV, Angermann BR, Wang JM, Kastenmuller W, et al. 2013. Neutrophil swarms require LTB4 and integrins at sites of cell death in vivo. Nature 498:371–75