Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 3
Abstract
A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.
Shown: (a) Rapid viral gene expression in SCS myeloid cells after MVA infection
Popliteal LNs from MVA NP-S-GFP(nuclear)-infected (footpad) animals were surgically exposed and analyzed by two-photon microscopy. Video shows onset of virally driven GFP expression in situ and identifies CD169+ myeloid cells as viral targets.
(b) Virally infected CD169+ myeloid cells actively move their fine processes
Popliteal LNs from MVA GFP(cytosolic)-infected (footpad) animals were surgically exposed and analyzed by two-photon microscopy. Video shows dynamic movement of infected GFP-expressing myeloid cells in situ.
Original publication: Kastenmüller W, Brandes M, Wang Z, Herz J, Egen JG, Germain RN. 2013. Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8+ T cell responses in the lymph node. Immunity 38:502–13