Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 4

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: CXCR6-GFP^bright cells are prepositioned near the LN capsule

A total of 3 × 106 CMTMR labeled naïve OT-1 T cells (red) were transferred into a CXCR6gfp/+ recipient. 24 h later the popliteal LN was surgically exposed and imaged using a two-photon microscope. The video shows a z stack (250 µm) scanned from the capsule to the paracortex of the LN.

Original publication: Kastenmüller W, Torabi-Parizi P, Subramanian N, Lammermann T, Germain RN. 2012. A spatially-organized multicellular innate immune response in lymph nodes limits systemic pathogen spread. Cell 150:1235–48