Retroviral Integrase: Then and Now: Video 1

A video from the 2015 review by Mark D. Andrake and Anna Marie Skalka, "Retroviral Integrase: Then and Now," from the Annual Review of Virology. 

Shown: Assembly of the prototype foamy virus (PFV) intasome (see Figure 3). This brief video portrays one possible scenario for how the PFV intasome may be assembled from PFV integrase (IN) monomers, the predominant multimer found in solution. The complex is shown in ribbon representation, with the inner subunits in red and blue. Viral DNA oligonucleotides are in orange ribbon ladder representation and the target DNA in yellow and black. The outer IN subunits only show the catalytic core domains and are colored gray. After the target DNA moves into place, the view zooms in to one active site to show the location of active site metals (depicted as green spheres) and the DDE residues (shown in stick representation) that are required for metal binding. This video was generated using UCSF Chimera software [Pettersen et al., J. Comput. Chem. 25:1605–12 (2004)] with the coordinates of PDB 4E7K.