Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 1

A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski, "Mechanics and Single-Molecule Interrogation of DNA Recombination," from the Annual Review of Biochemistry. 

Supplemental Video 1 Unwinding of a single molecule of λ DNA by RecBCD. 

This video shows unwinding of a single molecule of λ DNA – stained with YOYO-1, attached to a 1-μm polystyrene bead (stained nonspecifically by YOYO-1), and captured by an optical trap, by a single RecBCD enzyme at 37°C in the presence of 1 mM ATP; DNA is extend by solution flow from the bottom to the top. This molecule unwound at ∼440 bp/sec, and dissociated after ∼26,800 bp. Published with permissions from Reference 30.