Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 2
Abstract
A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski, "Mechanics and Single-Molecule Interrogation of DNA Recombination," from the Annual Review of Biochemistry.
Supplemental Video 2 Unwinding of a single molecule of DNA by RecBCD.
This video shows translocation of an individual RecBCD molecule along χ-containing λ DNA, visualized by virtue of a fluorescent 40-nm particle attached to a biotinylated RecD subunit. Solution flow is left to right. The very bright spot to the left is the 1-μm polystyrene bead, to which nanoparticles are bound nonspecifically, in the optical trap. Note that the nanoparticle--RecBCD complex can be seen to pause for about 6.6 s, and then it continues to translocate but at a much reduced speed (∼145 bp/sec versus ∼1,120 bp/sec prior to χ-recognition). Published with permission from Reference 54.