Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 5

A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski, "Mechanics and Single-Molecule Interrogation of DNA Recombination," from the Annual Review of Biochemistry.

Supplemental Video 5 Salt-induced intramolecular condensation of SSB-ssDNA imaged using TIRF microscopy.

Video of a single molecule of SSB^AF488-coated λ ssDNA, tethered at one end and imaged using TIRF microscopy, contracting in length upon increasing [NaOAc] from 0 to 100 mM. Solution flow is left to right. The video frames were rendered into a topological intensity map. Time zero corresponds to the time at which the pump was turned on. The dead time of the experiment was approximately 25 s due to the volume in the lines between the syringe valve and the microfluidic chamber. Published with permission from Reference 102.