Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 6

A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski, "Mechanics and Single-Molecule Interrogation of DNA Recombination," from the Annual Review of Biochemistry.

Supplemental Video 6 Condensation of SSB in the absence of free protein during a transient increase from 0 to 100 mM NaOAc.

Video of a single molecule of SSB^AF488-coated λ ssDNA contracting in length as the salt concentration is increased from 0 to 100 mM NaOAc, and then subsequently reduced back to zero mM, conducted in the absence of free SSB^AF488. Solution flow is left to right. The flow cell was extensively washed with buffer to remove free SSB protein before beginning the experiment. Video recording began when the pump was turned on, requiring ∼40-50 s for the dead volume to be flushed from the lines to the flow chamber. SSB^AF488 was omitted from both of the high-salt washes and from the 0 mM wash. Published with permission from Reference 102.