Mechanics and Single-Molecule Interrogation of DNA Recombination: Supplemental Video 10

A supplemental video from the 2016 review by Jason C. Bell and Stephen C. Kowalczykowski, "Mechanics and Single-Molecule Interrogation of DNA Recombination," from the Annual Review of Biochemistry.

Supplemental Video 10 Dissociation of heterologously paired RecA nucleoprotein filaments during a DNA pairing experiment.

Video, performed as in Supplemental Video 9, showing RecA nucleoprotein filaments, both heterologously bound and homologously bound (left and right red spots, respectively) during the extension step of a pairing assay performed using the 1,762 nt homologous ssDNA. As the beads are separated, several loop-release events are observed involving the heterologously bound filament (left) before its dissociation from λ DNA, whereas the homologously bound RecA nucleoprotein filament (right) remains stably bound. Published with permission from Reference 152.