Single-Cell Studies of Phage λ: Hidden Treasures Under Occam's Rug: Video 3

A video from the 2016 review by Ido Golding, "Single-Cell Studies of Phage λ: Hidden Treasures Under Occam’s Rug," from the Annual Review of Virology.

Shown: P_R activity during lysogen induction (see also Figure 2g ). In the reporter strain, mRNA from the P_R promoter is labeled using MS2-GFP (green), whereas the genome locus where the reporter resides is labeled using TetR-mCherry (red). The cells are λ lysogens. At t = 20 min, mitomycin C is added, leading to transient derepression of P_R and induction of the dormant prophage. The prophage used is lysis deficient; hence, the cells do not lyse at the end of the induction process. Video courtesy of Jing Zhang, Louis McLane, and Samuel Skinner; Golding lab (unpublished).