1932

Abstract

The Watson-Crick double helix of DNA was first revealed in 1953. Since then a wide range of physical chemical methods have been applied to DNA and to its more versatile relative RNA to determine their structures and functions. My major goal is to predict the folded structure of any RNA from its sequence. We have used bulk and single-molecule measurements of thermodynamics and kinetics, plus various spectroscopic methods (UV absorption, optical rotation, circular dichroism, circular intensity differential scattering, fluorescence, NMR) to approach this goal.

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/content/journals/10.1146/annurev.physchem.53.082001.144341
2002-10-01
2024-04-26
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Literature Cited

  1. Moffitt W, Fitts DD, Kirkwood JG. 1957. Theory of optical activity of helical polymers.. Proc. Natl. Acad. Sci. USA 43:723–30 [Google Scholar]
  2. Tinoco I Jr, Freeman MP. 1957. The optical activity of oriented copper helices.. I. Experimental J. Phys. Chem. 61:1196–200 [Google Scholar]
  3. Tinoco I Jr, Woody RW. 1964. Optical rotation of oriented helices.. IV. A free electron on a helix J. Chem. Phys. 40:160–65 [Google Scholar]
  4. Moore D, Tinoco I Jr. 1980. The circular dichroism of large helices, a free particle on a helix.. J. Chem. Phys. 82:3396–400 [Google Scholar]
  5. Townes CH. 1999. How the Laser Happened. New York: Oxford Univ. Press [Google Scholar]
  6. Tinoco I Jr. 1960. Hypochromism in polynucleotides.. J. Am. Chem. Soc. 82:4785–90 Erratum.; 1961. J. Am. Chem. Soc. 84:5047 The error in this article was an elementary one. The referees kept the manuscript about two months and then made only minor suggestions. I later thought that if they were going to read it so carefully, they should at least have found the mistake [Google Scholar]
  7. Tinoco I Jr, Uhlenbeck OC, Levine MD. 1971. Estimation of secondary structure in ribonucleic acids.. Nature 230:362–67 [Google Scholar]
  8. Tinoco I Jr, Borer PN, Dengler B, Levine MD, Uhlenbeck OC, Crothers DM, Gralla J. 1973. Improved estimation of secondary structure in ribonucleic acids.. Nat. New Biol. 246:40–41 [Google Scholar]
  9. Turner DH, Tinoco I Jr, Maestre MF. 1974. Fluorescence detected circular dichroism.. J. Am. Chem. Soc. 96:4340–42 [Google Scholar]
  10. Tinoco I Jr, Turner DH. 1976. Fluorescence detected circular dichroism.. Theory J. Am. Chem. Soc. 98:6453–56 [Google Scholar]
  11. Maestre MF, Bustamante C, Hayes TL, Subirana JA, Tinoco I Jr. 1982. Differential scattering of circularly polarized light by the helical sperm head from the octopus eledone cirrhosa.. Nature 298:773–74 [Google Scholar]
  12. Harris RA, Tinoco I Jr. 1994. Laser-perturbed NMR spectroscopy and the conservation of parity.. J. Chem. Phys. 101:9289–94 [Google Scholar]
  13. Dickerson RE. 1983. The DNA helix and how it is read.. Sci. Am. 249:94–98 [Google Scholar]
  14. Chen XY, Chamorro M, Lee SI, Shen LX, Hines JV, Tinoco I Jr, Varmus HE. 1995. Structural and functional studies of retroviral RNA pseudoknots involved in ribosomal frameshifting: Nucleotides at the junction of the two stems are important for efficient ribosomal frameshifting.. EMBO J. 14:842–52 There was some discussion about whether the first author should be from my lab or Varmus' lab. The compromise was that the first two authors have asterisks with the statement that both contributed equally to the work. I thought that Varmus and I should also have asterisks so we could equally share the desirable last position [Google Scholar]
  15. Liphardt J, Onoa B, Smith SB, Tinoco I Jr, Bustamante C. 2001. Reversible unfolding of a single RNA molecule by force.. Science 292:733–37 [Google Scholar]
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