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Annual Review of Physical Chemistry

Volume 66, 2015

Site-Specific Infrared Probes of Proteins

Abstract

Infrared spectroscopy has played an instrumental role in the study of a wide variety of biological questions. However, in many cases, it is impossible or difficult to rely on the intrinsic vibrational modes of biological molecules of interest, such as proteins, to reveal structural and environmental information in a site-specific manner. To overcome this limitation, investigators have dedicated many recent efforts to the development and application of various extrinsic vibrational probes that can be incorporated into biological molecules and used to site-specifically interrogate their structural or environmental properties. In this review, we highlight recent advancements in this rapidly growing research area.

Keyword(s): infrared spectroscopy, isotope editing, site-specific probe, unnatural amino acid, vibrational coupling, vibrational Stark effect spectroscopy
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/content/journals/10.1146/annurev-physchem-040214-121802
2015-04-01
2024-04-19
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Site-Specific Infrared Probes of Proteins
Annual Review of Physical Chemistry 66, 357 (2015); https://doi.org/10.1146/annurev-physchem-040214-121802
/content/journals/10.1146/annurev-physchem-040214-121802
/content/journals/10.1146/annurev-physchem-040214-121802
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