1932

Abstract

This chapter represents a travelog of my life and career and the philosophical points I acquired along the way. I was born on a sugar plantation on the island of Hawaii and early on had a stuttering problem. I attended the Kamehameha Schools and received my BS and MS degrees from the University of Hawaii and my Ph.D. from the University of California at Davis. I link my life and career to various principles and events, some of which are: the importance of positioning oneself; going for the big enchilada; music, the international language; the red zone of biotechnology; the human side of biotechnology; the transgenic papaya story; and my leadership time at USDA in Hawaii. The guiding light throughout my career were the words from Drs. Eduardo Trujillo and Robert Shepherd, respectively, “Dennis, don't just be a test tube scientist, do something to help people” and “Now tell me, what have you really accomplished?”

Loading

Article metrics loading...

/content/journals/10.1146/annurev-phyto-080614-120314
2015-08-04
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/phyto/53/1/annurev-phyto-080614-120314.html?itemId=/content/journals/10.1146/annurev-phyto-080614-120314&mimeType=html&fmt=ahah

Literature Cited

  1. Bar-Joseph M, Garnsey SM, Gonsalves D, Moscovitz M, Purcifull DE. 1.  et al. 1979. The use of enzyme-linked immunosorbent assay for detection of citrus tristeza virus. Phytopathology 69:190–94 [Google Scholar]
  2. Bitterlin MW, Gonsalves D. 2.  1988. Serological grouping of tomato ringspot virus isolates: implications for diagnosis and cross-protection. Phytopathology 78:278–85 [Google Scholar]
  3. Bol JF, Van Vloten-Doting L, Jaspars EMJ. 3.  1971. A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85 [Google Scholar]
  4. Davidson SN. 4.  2008. Forbidden fruit: transgenic papaya in Thailand. Plant Physiol. 147:487–93 [Google Scholar]
  5. Dawson WO, Bar-Joseph M, Garnsey SM, Moreno P. 5.  2015. Citrus tristeza virus: making an ally from an enemy. Annu. Rev. Phytopathol. 53:137–55 [Google Scholar]
  6. Edwards MC, Gonsalves D, Provvidenti R. 6.  1983. Genetic analysis of cucumber mosaic virus in relation to host resistance: location of determinants for pathogenicity to certain legumes and Lactuca saligna. Phytopathology 73:269–73 [Google Scholar]
  7. Fermin G, Keith RC, Suzuki JY, Ferreira S, Gaskill DA. 7.  et al. 2011. Allergenicity assessment of the Papaya ringspot virus coat protein expressed in transgenic Rainbow papaya. J. Agric. Food Chem. 59:10006–12 [Google Scholar]
  8. Ferreira SA, Mau RFL, Manshardt R, Pitz KY, Gonsalves D. 8.  1992. Field evaluation of papaya ringspot virus cross protection. Proc. Annu. Hawaii Papaya Ind. Assoc. Conf., 28th, Honolulu Sept. 25–26 14–19 Honolulu, HI: Univ. Hawaii [Google Scholar]
  9. Ferreira SA, Pitz KY, Manshardt R, Zee F, Fitch M, Gonsalves D. 9.  2002. Virus coat protein transgenic papaya provides practical control of papaya ringspot virus in Hawaii. Plant Dis. 86:101–5 [Google Scholar]
  10. Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC. 10.  1992. Virus resistant papaya derived from tissues bombarded with the coat protein gene of papaya ringspot virus. Nat. Biotechnol. 10:1466–72 [Google Scholar]
  11. Fuchs M, Gonsalves D. 11.  2007. Safety of virus-resistant transgenic plants two decades after their introductions: lessons from realistic field risk assessments studies. Annu. Rev. Phytopathol. 45:173–202 [Google Scholar]
  12. Fuchs M, McFerson JR, Tricoli DM, McMaster JR, Deng RZ. 12.  et al. 1997. Cantaloupe line CZW-30 containing coat protein genes of cucumber mosaic virus, zucchini yellow mosaic virus, and watermelon mosaic virus-2 is resistant to these aphid-borne viruses in the field. Mol. Breed. 3:279–90 [Google Scholar]
  13. Fuchs M, Provvidenti R, Slightom JL, Gonsalves D. 13.  1996. Evaluation of trangenic tomatoes expressing the coat protein gene of cucumber mosaic virus strain WL under field conditions. Plant Dis. 80:270–75 [Google Scholar]
  14. Fuchs M, Tricoli D, Carney DM, Schesser KJ, McFerson JR, Gonsalves D. 14.  1998. Comparative virus resistance and fruit yield of transgenic squash with single and multiple coat protein genes. Plant Dis. 82:1350–56 [Google Scholar]
  15. Garnsey SM, Gonsalves D, Purcifull DE. 15.  1979. Rapid diagnosis of citrus tristeza virus infections by sodium dodecyl sulfate-immunodiffusion procedures. Phytopathology 69:88–95 [Google Scholar]
  16. Gonsalves C, Lee D, Gonsalves D. 16.  2004. Transgenic virus resistant papaya: The Hawaiian “Rainbow” was rapidly adopted by farmers and is of major importance in Hawaii today. APSnet Features. doi:10.1094/APSnetFeature-2004-0804
  17. Gonsalves C, Lee DR, Gonsalves D. 17.  2007. The adoption of genetically modified papaya in Hawaii and its implications for developing countries. J. Dev. Stud. 43:177–91 [Google Scholar]
  18. Gonsalves CV, Gonsalves D. 18.  2014. The Hawaii papaya story. Handbook on Agriculture, Biotechnology and Development SJ Smyth, PWB Pillips, D Castle 642–60 Northhampton, MA: Edward Elgar Publ. [Google Scholar]
  19. Gonsalves D. 19.  1998. Control of papaya ringspot virus in papaya: a case study. Annu. Rev. Phytopathol. 36:415–37 [Google Scholar]
  20. Gonsalves D. 20.  2006. Transgenic papaya: development, release, impact, and challenges. Adv. Virus Res. 67:317–54 [Google Scholar]
  21. Gonsalves D. 21.  2014. Hawaii's transgenic papaya story 1978–2012: a personal account. Genetics and Genomics of Papaya R Ming, P Moore 115–42 New York: Springer [Google Scholar]
  22. Gonsalves D, Chee P, Provvidenti R, Seem R, Slightom JL. 22.  1992. Comparison of coat protein–mediated and genetically-derived resistance in cucumbers to infection by cucumber mosaic virus under field conditions with natural challenge inoculations by vectors. Nat. Biotechnol. 10:1562–70 [Google Scholar]
  23. Gonsalves D, Garnsey SM. 23.  1975. Functional equivalence of an RNA component and coat protein for infectivity of citrus leaf rugose virus. Virology 64:23–31 [Google Scholar]
  24. Gonsalves D, Gonsalves C, Carr J, Tripathi S, Matsumoto T. 24.  et al. 2012. Assaying for pollen drift from transgenic “Rainbow” to nontransgenic “Kapoho” papaya under commercial and experimental field conditions in Hawaii. Trop. Plant Biol. 5:153–60 [Google Scholar]
  25. Gonsalves D, Ishii M. 25.  1980. Purification and serology of papaya ringspot virus. Phytopathology 70:1028–32 [Google Scholar]
  26. Gonsalves D, Purcifull DE, Garnsey SM. 26.  1978. Purification and serology of citrus tristeza virus. Phytopathology 68:553–59 [Google Scholar]
  27. Gonsalves D, Shepherd RJ. 27.  1972. Biological and physical properties of the two nucleoprotein components of pea enation mosaic virus and their associated nucleic acids. Virology 48:709–23 [Google Scholar]
  28. Gonsalves D, Trujillo E, Hoch HC. 28.  1986. Purification and some properties of a virus associated with cardamom mosaic, a new member of the potyvirus group. Plant Dis. 70:65–69 [Google Scholar]
  29. Gonsalves D, Trujillo EE. 29.  1986. Tomato spotted wilt virus in papaya carica–papaya and detection of the virus by ELISA. Plant Dis. 70:501–6 [Google Scholar]
  30. Gonsalves D, Vegas A, Prasartsee V, Drew R, Suzuki J, Tripathi S. 30.  2006. Developing papaya to control papaya ringspot virus by transgenic resistance, intergeneric hybridization, and tolerance breeding. Plant Breed. Rev. 26:35–78 [Google Scholar]
  31. Kanahele G. 31.  1986. Pauahi: The Kamehameha Legacy Honolulu, HI: Kamehameha Schools Press223
  32. Lakshman DK, Gonsalves D. 32.  1985. Genetic analyses of two large-lesion isolates of cucumber mosaic virus. Phytopathology 75:758–62 [Google Scholar]
  33. Ling K, Zhu H, Gonsalves D. 33.  2004. Complete nucleotide sequence and genome organization of grapevine leafroll associated virus 3, type member of the genus Ampelovirus. J. Gen. Virol. 85:2099–102 [Google Scholar]
  34. Lius S, Manshardt RM, Fitch MMM, Slightom JL, Sanford JC, Gonsalves D. 34.  1997. Pathogen-derived resistance provides papaya with effective protection against papaya ringspot virus. Mol. Breed. 3:161–68 [Google Scholar]
  35. Lynas M, Evanega SD. 35.  2014. The dialectic of pro-poor papaya. The Oxford Handbook of Food, Politics, and Society RJ Herring Oxford, UK: Oxford Univ. Press [Google Scholar]
  36. Manshardt RM. 36.  1998. “UH Rainbow” papaya. New Plants Hawaii 1:1–2 [Google Scholar]
  37. Mau RFL, Gonsalves D, Bautista R. 37.  1989. Use of cross protection to control papaya ringspot virus at Waianae. Proc. Annu. Papaya Ind. Assoc. Conf., 25th, Hilo Sept. 29–30 77–84 Honolulu, HI: Univ. Hawaii [Google Scholar]
  38. Meng BZ, Pang SZ, Forsline PL, McFerson JR, Gonsalves D. 38.  1998. Nucleotide sequence and genome structure of grapevine Rupestris stem pitting associated virus-1 reveal similarities to apple stem pitting virus. J. Gen. Virol. 79:2059–69 [Google Scholar]
  39. Ming R, Hou S, Feng Y, Yu Q, Dionne-Laporte A. 39.  et al. 2008. A draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus). Nature 452:991–96 [Google Scholar]
  40. Pang SZ, Jan FJ, Carney K, Stout J, Tricoli DM. 40.  et al. 1996. Post-transcriptional transgene silencing and consequent tospovirus resistance in transgenic lettuce are affected by transgene dosage and plant development. Plant J. 9:899–909 [Google Scholar]
  41. Pang SZ, Jan FJ, Gonsalves D. 41.  1997. Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants. Proc. Natl. Acad. Sci. USA 94:8261–66 [Google Scholar]
  42. Pang SZ, Jan FJ, Tricoli DM, Russell PF, Carney KJ. 42.  et al. 2000. Resistance to squash mosaic comovirus in transgenic squash plants expressing its coat protein genes. Mol. Breed. 6:87–93 [Google Scholar]
  43. Pitz K, Ferreira S, Mau R, Gonsalves D. 43.  1994. Papaya cross protection: the near-commercialization experience on Oahu. Proc. Annu. Hawaii Papaya Ind. Assoc. Conf., 30th, Maui Sept. 23–24 4–6 Honolulu, HI: Univ. Hawaii [Google Scholar]
  44. Powell-Abel P, Nelson RS, De B, Hoffmann N, Rogers SG. 44.  et al. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232:738–43 [Google Scholar]
  45. Provvidenti R, Gonsalves D. 45.  1982. Resistance to papaya ringspot virus in Cucumis metuliferus and its relationship to resistance to watermelon mosaic virus 1. J. Hered. 73:239–40 [Google Scholar]
  46. Sakuanrungrsirikul S, Sarindu N, Prasartsee V, Chaikiatiyos S, Siriyan R. 46.  et al. 2005. Update on the development of virus-resistant papaya: virus-resistant transgenic papaya for people in rural communities of Thailand. Food Nutr. Bull. 26:422–26 [Google Scholar]
  47. Sanford JC, Johnston SA. 47.  1985. The concept of parasite-derived resistance: deriving resistance genes from the parasite's own genome. J. Theor. Biol. 113:395–405 [Google Scholar]
  48. Schmitt RC. 48.  1968. Demographic Statistics of Hawaii, 1778–1965 Honolulu, HI: Univ. Hawaii Press271
  49. Smith KM. 49.  1957. A Textbook of Plant Virus Diseases Boston, MA: Little, Brown
  50. Suzuki JY, Tripathi S, Fermin GA, Jan F-J, Hou S. 50.  et al. 2008. Characterization of inserton sites in Rainbow papaya, the first commercialized transgenic papaya fruit crop. Trop. Plant Biol. 1:293–309 [Google Scholar]
  51. Taiwo MA, Gonsalves D, Provvidenti R, Thurston HD. 51.  1982. Partial characterization and grouping of isolates of blackeye cowpea mosaic and cowpea aphidborne mosaic viruses. Phytopathology 72:590–96 [Google Scholar]
  52. Tennant PF, Gonsalves C, Ling KS, Fitch M, Manshardt R. 52.  et al. 1994. Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya. Phytopathology 84:1359–66 [Google Scholar]
  53. Tripathi S, Suzuki JY, Carr JB, McQuate GT, Ferreira SA. 53.  et al. 2011. Nutritional composition of Rainbow papaya, the first commercialized transgenic fruit crop. J. Food Compos. Anal. 24:140–47 [Google Scholar]
  54. Vargas RI, Mau RFL, Jang EB, Faust RM, Wong L. 54.  2008. The Hawaii fruit fly area-wide pest management program. Areawide Pest Management: Theory and Implementation O Koul, GW Cuperus, NC Elliott 300–25 London: CABI [Google Scholar]
  55. Wang HL, Gonsalves D, Provvidenti R, Lecoq HL. 55.  1991. Effectiveness of cross protection by a mild strain of zucchini yellow mosaic virus in cucumber melon and squash. Plant Dis. 75:203–7 [Google Scholar]
  56. Wang HL, Yeh S-D, Chiu RJ, Gonsalves D. 56.  1987. Effectiveness of cross-protection by mild mutants of papaya ringspot virus for control of ringspot disease of papaya in Taiwan. Plant Dis. 71:491–97 [Google Scholar]
  57. Wang M, Gonsalves D. 57.  1992. Artificial induction and evaluation of a mild isolate of tomato spotted wilt virus. J. Phytopathol. 135:233–44 [Google Scholar]
  58. Xue B, Gonsalves C, Provvidenti R, Slightom JL, Fuchs M, Gonsalves D. 58.  1994. Development of transgenic tomato expressing a high level of resistance to cucumber mosaic virus strains of subgroups I and II. Plant Dis. 78:1038–41 [Google Scholar]
  59. Yeh S-D, Gonsalves D. 59.  1994. Practices and perspective of control of papaya ringspot virus by cross protection. Advances in Disease Vector Research KF Harris 237–57 New York: Springer-Verlag [Google Scholar]
  60. Yeh S-D, Gonsalves D. 60.  1984. Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74:1086–91 [Google Scholar]
  61. Yeh S-D, Gonsalves D, Wang H-L, Namba R, Chiu R-J. 61.  1988. Control of papaya ringspot virus by cross protection. Plant Dis 72:375–80 [Google Scholar]
  62. Zee F, Gonsalves D, Goheen A, Kim KS, Pool R, Lee RF. 62.  1987. Cytopathology of leafroll-diseased grapevines and the purification and serology of associated closteroviruslike particles. Phytopathology 77:1427–34 [Google Scholar]
  63. Zhu HY, Ling KS, Goszczynski DE, McFerson J, Gonsalves D. 63.  1998. Nucleotide sequence and genome organization of grapevine leafroll associated closterovirus-2 is similar to beet yellows virus, the closterovirus type member. J. Gen. Virol. 79:1289–98 [Google Scholar]
/content/journals/10.1146/annurev-phyto-080614-120314
Loading
/content/journals/10.1146/annurev-phyto-080614-120314
Loading

Data & Media loading...

  • Article Type: Review Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error