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Annual Review of Analytical Chemistry

Volume 18, 2025

Protein Engineering for Spatiotemporally Resolved Cellular Monitoring

  • Guanwei Zhou1 and Wenjing Wang1,2
  • 1Life Sciences Institute and Program in Chemical Biology, University of Michigan, Ann Arbor, Michigan, USA; email: [email protected], [email protected] 2Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA
  • Vol. 18:217-240 (Volume publication date May 2025)
  • First published as a Review in Advance on February 25, 2025
  • Copyright © 2025 by the author(s).
    This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See credit lines of images or other third-party material in this article for license information.

Abstract

Protein engineering has been extensively applied to the development of genetically encoded reporters for spatiotemporally resolved monitoring of dynamic biochemical activity across cellular compartments in living cells. Genetically encoded reporters facilitate the visualization and recording of cellular processes, including transmission of signaling molecules, protease activity, and protein–protein interactions. In this review, we describe and assess common reporter motifs and protein engineering strategies for designing genetically encoded reporters. We also discuss essential parameters for evaluating genetically encoded reporters, along with future protein engineering opportunities in this field.

Keyword(s): genetically encoded reporters, protease activity, protein engineering, protein–protein interactions, signaling molecules, spatiotemporal resolution
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2025-05-15
2025-06-15
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Protein Engineering for Spatiotemporally Resolved Cellular Monitoring
Annual Review of Analytical Chemistry 18, 217 (2025); https://doi.org/10.1146/annurev-anchem-070124-035857
/content/journals/10.1146/annurev-anchem-070124-035857
/content/journals/10.1146/annurev-anchem-070124-035857
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