1932

Abstract

When first asked to write a review of my life as a scientist, I doubted anyone would be interested in reading it. In addition, I did not really want to compose my own memorial. However, after discussing the idea with other scientists who have written autobiographies, I realized that it might be fun to dig into my past and to reflect on what has been important for me, my life, my family, my friends and colleagues, and my career. My life and research has taken me from bacteriophage to –mediated DNA transfer to plants to the plant genome and its environmentally induced changes. I went from being a naïve, young student to a postdoc and married mother of two to the leader of an ever-changing group of fantastic coworkers—a journey made rich by many interesting scientific milestones, fascinating exploration of all corners of the world, and marvelous friendships.

Loading

Article metrics loading...

/content/journals/10.1146/annurev-arplant-050718-100143
2019-04-29
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/arplant/70/1/annurev-arplant-050718-100143.html?itemId=/content/journals/10.1146/annurev-arplant-050718-100143&mimeType=html&fmt=ahah

Literature Cited

  1. 1.  Bakkeren G, Koukolíková-Nicola Z, Grimsley N, Hohn B 1989. Recovery of Agrobacterium tumefaciens T-DNA molecules from whole plants early after transfer. Cell 57:847–57
    [Google Scholar]
  2. 2.  Benzer S 1955. Fine structure of a genetic region in bacteriophage. PNAS 41:344–54
    [Google Scholar]
  3. 3.  Boyko A, Blevins T, Yao Y, Golubov A, Bilichak A et al. 2010. Transgenerational adaptation of Arabidopsis to stress requires DNA methylation and the function of Dicer-like proteins. PLOS ONE 5:e9514
    [Google Scholar]
  4. 4.  Citovsky V, Zupan J, Warnick D, Zambryski P 1992. Nuclear localization of Agrobacterium VirE2 protein in plant cells. Science 256:1802–5
    [Google Scholar]
  5. 5.  Collins J, Hohn B 1978. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage λ heads. PNAS 75:4242–46
    [Google Scholar]
  6. 6.  Das OP, Levi-Minzi S, Benner M, Messing J 1990. A somatic gene rearrangement contributing to genetic diversity in maize. PNAS 87:7809–13
    [Google Scholar]
  7. 7.  Dawson P, Hohn B, Hohn T, Skalka A 1976. Functional empty capsid precursors produced by lambda mutant defective for late λ DNA replication. J. Virol. 17:576–83
    [Google Scholar]
  8. 8.  Dürrenberger F, Crameri A, Hohn B, Koukolíková-Nicola Z 1989. Covalently bound VirD2 protein of Agrobacterium tumefaciens protects the T-DNA from exonucleolytic degradation. PNAS 86:9154–58
    [Google Scholar]
  9. 9.  Endo M, Ishikawa Y, Osakabe K, Nakayama S, Kaya H et al. 2006. Increased frequency of homologous recombination and T-DNA integration in Arabidopsis CAF-1 mutants. EMBO J 25:5579–90
    [Google Scholar]
  10. 10.  Franck A, Guilley H, Jonard G, Richards K, Hirth L 1980. Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–94
    [Google Scholar]
  11. 11.  Fritsch O, Benvenuto G, Bowler C, Molinier J, Hohn B 2004. The INO80 protein controls homologous recombination in Arabidopsis thaliana. Mol. Cell 16:479–85
    [Google Scholar]
  12. 12.  Gelvin SB 2017. Integration of Agrobacterium T-DNA into the plant genome. Annu. Rev. Genet. 51:195–217
    [Google Scholar]
  13. 13.  Georgopoulos CP, Hohn B 1978. Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product). PNAS 75:131–35
    [Google Scholar]
  14. 14.  Gierer A, Schramm G 1956. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature 177:702–3
    [Google Scholar]
  15. 15.  Gietl C, Koukolíková-Nicola Z, Hohn B 1987. Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA. PNAS 84:9006–10
    [Google Scholar]
  16. 16.  Grimsley N, Hohn B, Hohn T, Walden R 1986. “Agroinfection,” an alternative route for viral infection of plants by using the Ti plasmid. PNAS 83:3282–86
    [Google Scholar]
  17. 17.  Grimsley N, Hohn T, Davies J, Hohn B 1987. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325:177–79
    [Google Scholar]
  18. 18.  Grimsley N, Hohn T, Hohn B 1986. Recombination in a plant virus: template-switching in cauliflower mosaic virus. EMBO J 5:641–46
    [Google Scholar]
  19. 19.  Hiei Y, Ishida Y, Komari T 2014. Progress of cereal transformation technology mediated by Agrobacterium tumefaciens. Front. Plant Sci. 5:628
    [Google Scholar]
  20. 20.  Hohn B 1983. DNA sequences necessary for packaging of bacteriophage λ DNA. PNAS 80:7456–60
    [Google Scholar]
  21. 21.  Hohn B, Balazs E, Ruegg D, Hohn T 1986. Splicing of an intervening sequence from hybrid cauliflower mosaic viral RNA. EMBO J 5:2759–62
    [Google Scholar]
  22. 22.  Hohn B, Collins J 1988. Ten years of cosmids. Trends Biotechnol 6:293–98
    [Google Scholar]
  23. 23.  Hohn B, Hohn T 1974. Activity of empty, headlike particles for packaging of DNA of bacteriophage λ in vitro. PNAS 71:2372–76
    [Google Scholar]
  24. 24.  Hohn B, Lechner H, Marvin DA 1971. Filamentous bacterial viruses. I. DNA synthesis during the early stages of infection with fd. J. Mol. Biol. 56:143–54
    [Google Scholar]
  25. 25.  Hohn B, Murray K 1977. Packaging recombinant DNA molecules into bacteriophage particles in vitro. PNAS 74:3259–63
    [Google Scholar]
  26. 26.  Hohn T 2013. Plant pararetroviruses: interactions of cauliflower mosaic virus with plants and insects. Curr. Opin. Virol. 3:629–38
    [Google Scholar]
  27. 27.  Hohn T, Hohn B, Lesot A, Lebeurier G 1980. Restriction map of native and cloned cauliflower mosaic virus DNA. Gene 11:21–31
    [Google Scholar]
  28. 28.  Hohn T, Rothnie H 2013. Plant pararetroviruses: replication and expression. Curr. Opin. Virol. 3:621–28
    [Google Scholar]
  29. 29.  Hohn T, Wurtz M, Hohn B 1976. Capsid transformation during packaging of bacteriophage λ DNA. Philos. Trans. R. Soc. B 276:51–61
    [Google Scholar]
  30. 30.  Kado CI 2014. Historical account on gaining insights on the mechanism of crown gall tumorigenesis induced by Agrobacterium tumefaciens. Front. Microbiol 5:340
    [Google Scholar]
  31. 31.  Kaneko T, Moisyadi S, Suganuma R, Hohn B, Yanagimachi R, Pelczar P 2005. Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection. Theriogenology 64:1704–15
    [Google Scholar]
  32. 32.  Koukolíková-Nicola Z, Shillito R, Hohn B, Wang K, Van Montagu M, Zambryski P 1985. Involvement of circular intermediates in the transfer of T-DNA from Agrobacterium tumefaciens to plant cells. Nature 313:191–96
    [Google Scholar]
  33. 33.  Kovalchuk I, Kovalchuk O, Arkhipov A, Hohn B 1998. Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident. Nat. Biotechnol. 16:1054–59
    [Google Scholar]
  34. 34.  Kovalchuk I, Kovalchuk O, Kalck V, Boyko V, Filkowski J et al. 2003. Pathogen-induced systemic plant signal triggers DNA rearrangements. Nature 423:760–62
    [Google Scholar]
  35. 35.  Kovalchuk O, Titov V, Hohn B, Kovalchuk I 2001. A sensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat. Biotechnol. 19:568–72
    [Google Scholar]
  36. 36.  Lebeurier G, Hirth L, Hohn B, Hohn T 1982. In vivo recombination of cauliflower mosaic virus DNA. PNAS 79:2932–36
    [Google Scholar]
  37. 37.  Lebeurier G, Hirth L, Hohn T, Hohn B 1980. Infectivities of native and cloned DNA of cauliflower mosaic virus. Gene 12:139–46
    [Google Scholar]
  38. 38.  Lenhard-Schuller R, Hohn B, Brack C, Hirama M, Tonegawa S 1978. DNA clones containing mouse immunoglobulin κ chain genes isolated by in vitro packaging into phage λ coats. PNAS 75:4709–13
    [Google Scholar]
  39. 39.  Levy AA 2016. T-DNA integration: Pol θ controls T-DNA integration. Nat. Plants 2:16170
    [Google Scholar]
  40. 40.  Lucht JM, Mauch-Mani B, Steiner HY, Metraux JP, Ryals J, Hohn B 2002. Pathogen stress increases somatic recombination frequency in Arabidopsis. Nat. Genet. 30:311–14
    [Google Scholar]
  41. 41.  Marillonnet S, Thoeringer C, Kandzia R, Klimyuk V, Gleba Y 2005. Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23:718–23
    [Google Scholar]
  42. 42.  Marvin DA, Hohn B 1969. Filamentous bacterial viruses. Bacteriol. Rev. 33:172–209
    [Google Scholar]
  43. 43.  Marvin DA, Symmons MF, Straus SK 2014. Structure and assembly of filamentous bacteriophages. Prog. Biophys. Mol. Biol. 114:80–122
    [Google Scholar]
  44. 44.  Mauch-Mani B, Baccelli I, Luna E, Flors V 2017. Defense priming: an adaptive part of induced resistance. Annu. Rev. Plant Biol. 68:485–512
    [Google Scholar]
  45. 45.  Mayerhofer R, Koncz-Kalman Z, Nawrath C, Bakkeren G, Crameri A et al. 1991. T-DNA integration: a mode of illegitimate recombination in plants. EMBO J 10:697–704
    [Google Scholar]
  46. 46.  McClintock B 1984. The significance of responses of the genome to challenge. Science 226:792–801
    [Google Scholar]
  47. 47.  Molinier J, Ramos C, Fritsch O, Hohn B 2004. CENTRIN2 modulates homologous recombination and nucleotide excision repair in Arabidopsis. Plant Cell 16:1633–43
    [Google Scholar]
  48. 48.  Molinier J, Ries G, Zipfel C, Hohn B 2006. Transgeneration memory of stress in plants. Nature 442:1046–49
    [Google Scholar]
  49. 49.  Nester EW 2014. Agrobacterium: nature's genetic engineer. Front. Plant Sci. 5:730
    [Google Scholar]
  50. 50.  Nester EW, Gordon MP, Kerr A, eds. 2005. Agrobacterium tumefaciens: From Plant Pathology to Biotechnology St. Paul, MN: APS
  51. 50a.  Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, et al. 1984.Direct gene transfer to plants EMBO J. 3:2717–22
  52. 51.  Pecinka A, Rosa M, Schikora A, Berlinger M, Hirt H et al. 2009. Transgenerational stress memory is not a general response in Arabidopsis. PLOS ONE 4:e5202
    [Google Scholar]
  53. 52.  Pelczar P, Kalck V, Gomez D, Hohn B 2004. Agrobacterium proteins VirD2 and VirE2 mediate precise integration of synthetic T-DNA complexes in mammalian cells. EMBO Rep 5:632–37
    [Google Scholar]
  54. 53.  Pfeiffer P, Hohn T 1983. Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–89
    [Google Scholar]
  55. 54.  Potrykus I 2015. From the concept of totipotency to biofortified cereals. Annu. Rev. Plant Biol. 66:1–22
    [Google Scholar]
  56. 55.  Puchta H, Dujon B, Hohn B 1996. Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination. PNAS 93:5055–60
    [Google Scholar]
  57. 56.  Puchta H, Hohn B 2012. In planta somatic homologous recombination assay revisited: a successful and versatile, but delicate tool. Plant Cell 24:4324–31
    [Google Scholar]
  58. 57.  Puchta H, Swoboda P, Gal S, Blot M, Hohn B 1995. Somatic intrachromosomal homologous recombination events in populations of plant siblings. Plant Mol. Biol. 28:281–92
    [Google Scholar]
  59. 58.  Puchta H, Swoboda P, Hohn B 1995. Induction of intrachromosomal homologous recombination in whole plants. Plant J 7:203–10
    [Google Scholar]
  60. 59.  Ries G, Heller W, Puchta H, Sandermann H, Seidlitz HK, Hohn B 2000. Elevated UV-B radiation reduces genome stability in plants. Nature 406:98–101
    [Google Scholar]
  61. 60.  Rossi L, Hohn B, Tinland B 1993. The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plant. Mol. Gen. Genet. 239:345–53
    [Google Scholar]
  62. 61.  Rossi L, Hohn B, Tinland B 1996. Integration of complete transferred DNA units is dependent on the activity of virulence E2 protein of Agrobacterium tumefaciens. PNAS 93:126–30
    [Google Scholar]
  63. 62.  Schuermann D, Fritsch O, Lucht JM, Hohn B 2009. Replication stress leads to genome instabilities in Arabidopsis DNA polymerase δ mutants. Plant Cell 21:2700–14
    [Google Scholar]
  64. 63.  Schuermann D, Molinier J, Fritsch O, Hohn B 2005. The dual nature of homologous recombination in plants. Trends Genet 21:172–81
    [Google Scholar]
  65. 64.  Shen WH, Ramos C, Hohn B 1998. Excision of Ds1 from the genome of maize streak virus in response to different transposase-encoding genes. Plant Mol. Biol. 36:387–92
    [Google Scholar]
  66. 65.  Skalka AMA 2017. Finding, conducting, and nurturing science: a virologist's memoir. Annu. Rev. Virol. 4:1–35
    [Google Scholar]
  67. 66.  Slaughter A, Daniel X, Flors V, Luna E, Hohn B, Mauch-Mani B 2012. Descendants of primed Arabidopsis plants exhibit resistance to biotic stress. Plant Physiol 158:835–43
    [Google Scholar]
  68. 67.  Stachel SE, Timmerman B, Zambryski P 1986. Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cells. Nature 322:706–12
    [Google Scholar]
  69. 68.  Swoboda P, Gal S, Hohn B, Puchta H 1994. Intrachromosomal homologous recombination in whole plants. EMBO J 13:484–89
    [Google Scholar]
  70. 69.  Tinland B, Hohn B, Puchta H 1994. Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. PNAS 91:8000–4
    [Google Scholar]
  71. 70.  Tinland B, Koukolíková-Nicola Z, Hall MN, Hohn B 1992. The T-DNA-linked VirD2 protein contains two distinct functional nuclear localization signals. PNAS 89:7442–46
    [Google Scholar]
  72. 71.  Tinland B, Schoumacher F, Gloeckler V, Bravo-Angel AM, Hohn B 1995. The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome. EMBO J 14:3585–95
    [Google Scholar]
  73. 72.  van Kregten M, de Pater S, Romeijn R, van Schendel R, Hooykaas PJ, Tijsterman M 2016. T-DNA integration in plants results from polymerase-θ-mediated DNA repair. Nat. Plants 2:16164
    [Google Scholar]
  74. 73.  Van Montagu M 2011. It is a long way to GM agriculture. Annu. Rev. Plant Biol. 62:1–23
    [Google Scholar]
  75. 74.  Watson JD, Crick FH 1953. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature 171:737–38
    [Google Scholar]
  76. 75.  Ziemienowicz A, Gorlich D, Lanka E, Hohn B, Rossi L 1999. Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium. PNAS 96:3729–33
    [Google Scholar]
  77. 76.  Ziemienowicz A, Merkle T, Schoumacher F, Hohn B, Rossi L 2001. Import of Agrobacterium T-DNA into plant nuclei: two distinct functions of VirD2 and VirE2 proteins. Plant Cell 13:369–83
    [Google Scholar]
  78. 77.  Ziemienowicz A, Tinland B, Bryant J, Gloeckler V, Hohn B 2000. Plant enzymes but not Agrobacterium VirD2 mediate T-DNA ligation in vitro. Mol. Cell. Biol. 20:6317–22
    [Google Scholar]
/content/journals/10.1146/annurev-arplant-050718-100143
Loading
/content/journals/10.1146/annurev-arplant-050718-100143
Loading

Data & Media loading...

  • Article Type: Review Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error