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Annual Review of Pharmacology and Toxicology

Volume 61, 2021

Signaling Microdomains in the Spotlight: Visualizing Compartmentalized Signaling Using Genetically Encoded Fluorescent Biosensors

Abstract

How cells muster a network of interlinking signaling pathways to faithfully convert diverse external cues to specific functional outcomes remains a central question in biology. Through their ability to convert dynamic biochemical activities to rapid and precise optical readouts, genetically encoded fluorescent biosensors have become instrumental in unraveling the molecular logic controlling the specificity of intracellular signaling. In this review, we discuss how the use of genetically encoded fluorescent biosensors to visualize dynamic signaling events within their native cellular context is elucidating the different strategies employed by cells to organize signaling activities into discrete compartments, or signaling microdomains, to ensure functional specificity.

Keyword(s): FRET, live-cell imaging, signal transduction, spatiotemporal dynamics
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/content/journals/10.1146/annurev-pharmtox-010617-053137
2021-01-06
2024-04-18
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/content/journals/10.1146/annurev-pharmtox-010617-053137
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Signaling Microdomains in the Spotlight: Visualizing Compartmentalized Signaling Using Genetically Encoded Fluorescent Biosensors
Annual Review of Pharmacology and Toxicology 61, 587 (2021); https://doi.org/10.1146/annurev-pharmtox-010617-053137
/content/journals/10.1146/annurev-pharmtox-010617-053137
/content/journals/10.1146/annurev-pharmtox-010617-053137
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