Annual Review of Biochemistry
Vol. 46:331-358 (Volume publication date July 1977)
https://doi.org/10.1146/annurev.bi.46.070177.001555
Shenghong Ma, Zhipeng Meng, Rui Chen, Kun-Liang Guan
Vol. 88, 2019
The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Figure 1: Core components of the Hippo pathway in mammalian cells. The Hippo pathway activity controls the dynamic localization of YAP/TAZ between nucleus and cytoplasm. (a) When the Hippo pathway is ...
Figure 2: Mechanical cues regulate YAP/TAZ activities through Rho GTPase and the Hippo pathway. Cell–cell contact, cell stretching, cell tension, extracellular matrix (ECM) stiffness, and changes in c...
Figure 3: The Hippo pathway integrates multiple signals to regulate the activity of YAP/TAZ. The LATS kinase activity is controlled by cell polarity and cell adhesion through KIBRA/NF2, tight junction...
Figure 4: YAP/TAZ and cancer hallmarks. The Hippo pathway effectors YAP/TAZ directly or indirectly control multiple cancer hallmarks, including proliferation, survival, evading growth suppressors, rep...
Haifeng Wang, Marie La Russa, Lei S. Qi
Vol. 85, 2016
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-...Read More
Figure 1: CRISPR-associated protein 9 (Cas9)-mediated sequence-specific genomic editing. (a) Comparison of programmable sequence-specific genome editing nucleases. (Top) Zinc-finger nucleases (ZFNs) a...
Figure 2: Nuclease-deactivated Cas9 (dCas9)-mediated sequence-specific gene regulation. (a) CRISPR interference (CRISPRi) strategies. Repression can occur with dCas9 alone in bacteria, which stericall...
Figure 3: Applications of CRISPR-associated protein 9 (Cas9) in gene editing, gene regulation, epigenome editing, and genomic imaging. (a) Cas9-mediated site-specific genome editing has a variety of a...
Nils Wiedemann and Nikolaus Pfanner
Vol. 86, 2017
Mitochondria are essential organelles with numerous functions in cellular metabolism and homeostasis. Most of the >1,000 different mitochondrial proteins are synthesized as precursors in the cytosol and are imported into mitochondria by five transport ...Read More
Figure 1: Overview of the five major protein import pathways of mitochondria. Presequence-carrying preproteins are imported by the translocase of the outer mitochondrial membrane (TOM) and the presequ...
Figure 2: The presequence pathway into the mitochondrial inner membrane (IM) and matrix. The translocase of the outer membrane (TOM) consists of three receptor proteins, the channel-forming protein To...
Figure 3: Role of the oxidase assembly (OXA) translocase in protein sorting. Proteins synthesized by mitochondrial ribosomes are exported into the inner membrane (IM) by the OXA translocase; the ribos...
Figure 4: Carrier pathway into the inner membrane. The precursors of the hydrophobic metabolite carriers are synthesized without a cleavable presequence. The precursors are bound to cytosolic chaperon...
Figure 5: Mitochondrial intermembrane space import and assembly (MIA) machinery. Many intermembrane space (IMS) proteins contain characteristic cysteine motifs. The precursors are kept in a reduced an...
Figure 6: Biogenesis of β-barrel proteins of the outer mitochondrial membrane. The precursors of β-barrel proteins are initially imported by the translocase of the outer membrane (TOM), bind to small ...
Figure 7: The dual role of mitochondrial distribution and morphology protein 10 (Mdm10) in protein assembly and organelle contact sites. Mdm10 associates with the sorting and assembly machinery (SAM) ...
Figure 8: Multiple import pathways for integral α-helical proteins of the mitochondrial outer membrane. The precursors of proteins with an N-terminal signal anchor sequence are typically inserted into...
Figure 9: The mitochondrial contact site and cristae organizing system (MICOS) interacts with protein translocases. MICOS consists of two core subunits, Mic10 and Mic60. Mic10 forms large oligomers th...
D. Michiel Pegtel and Stephen J. Gould
Vol. 88, 2019
Exosomes are small, single-membrane, secreted organelles of ∼30 to ∼200 nm in diameter that have the same topology as the cell and are enriched in selected proteins, lipids, nucleic acids, and glycoconjugates. Exosomes contain an array of membrane-...Read More
Figure 1: Exosomes are heterogeneous in size, heterogeneous in composition, and enriched in membrane-associated, high-order oligomeric protein complexes. Although it is likely that no exosome contains...
Figure 2: Exosomes bud from endosome and plasma membranes. Exosome biogenesis occurs by three modes: () vesicle budding into discrete endosomes that mature into multivesicular bodies, which release ex...
Roger Y. Tsien
Vol. 67, 1998
In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a ...Read More
Figure 1: GFP sequences. (Line 1) The wild-type (WT) gfp10 gene as originally cloned and sequenced by Prasher et al (10). (Line 2) A popular humanized version (EGFP, Clontech Laboratories, Palo Alto,...
Figure 2: Mechanism proposed by Cubitt et al (22) for the intramolecular biosynthesis of the GFP chromophore, with rate constants estimated for the Ser65 → Thr mutant by Reid & Flynn (23) and Heim et...
Figure 3: Stereoview of the three-dimensional structure of GFP (30), showing 11 β-strands forming a hollow cylinder through which is threaded a helix bearing the chromophore, shown in ball-and-stick ...
Figure 4: Amino acid side chains, main chain carbonyls and amides, and solvent waters in the immediate vicinity of the chromophore of S65T GFP (30). Side chains are labeled with the one-letter code f...
Figure 5: Fluorescence excitation and emission spectra (solid and dashed lines, respectively) for typical members of the six major classes of GFP mutants, together with the chromophore structures bel...
Figure 6: Location on the GFP crystal structure (30) of the most important sites that improve folding at 37°C. The amino acids shown in space-filling representation are the wild-type residues that ar...
