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Annual Review of Analytical Chemistry

Volume 5, 2012

My Life with LIF: A Personal Account of Developing Laser-Induced Fluorescence

Abstract

Laser-induced fluorescence (LIF) is a spectroscopic technique that involves the excitation of a molecular target by a beam of laser radiation followed by the detection of the subsequent emission of radiation from the target. LIF detection has several advantages over absorption spectroscopy. First, LIF has excellent detection sensitivity because a signal is observed against a dark background. Second, the emitted radiation can be collected at various angles with respect to the incoming laser beam, making it possible to obtain two- and three-dimensional images because the fluorescence is emitted in all directions. Third, by dispersing the fluorescence, it is also possible to learn about the transitions from the state excited to the various lower levels of the target species. Finally, because of the delay between the excitation and detection events, it is also possible to learn about what processes the excited target undergoes in the intervening time.

Keyword(s): Autobiography, intensity, internal state distribution, lifetime, molecular structure, reaction dynamics, single-molecule spectroscopy

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My Life with LIF: A Personal Account of Developing Laser-Induced Fluorescence: Supplemental Video 1
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2012-07-19
2024-04-23
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My Life with LIF: A Personal Account of Developing Laser-Induced Fluorescence
Annual Review of Analytical Chemistry 5, 1 (2012); https://doi.org/10.1146/annurev-anchem-062011-143148
/content/journals/10.1146/annurev-anchem-062011-143148
/content/journals/10.1146/annurev-anchem-062011-143148
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Supplemental Material

Supplementary Data

  • Supplemental Video 1: The author illustrates laser-induced fluorescence of I2 excited with a green laser pointer. Download video file (WMV)

  • Article Type: Review Article

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