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Annual Review of Biophysics

Volume 47, 2018

Serial Femtosecond Crystallography of G Protein–Coupled Receptors

Abstract

G protein–coupled receptors (GPCRs) represent a large superfamily of membrane proteins that mediate cell signaling and regulate a variety of physiological processes in the human body. Structure-function studies of this superfamily were enabled a decade ago by multiple breakthroughs in technology that included receptor stabilization, crystallization in a membrane environment, and microcrystallography. The recent emergence of X-ray free-electron lasers (XFELs) has further accelerated structural studies of GPCRs and other challenging proteins by overcoming radiation damage and providing access to high-resolution structures and dynamics using micrometer-sized crystals. Here, we summarize key technology advancements and major milestones of GPCR research using XFELs and provide a brief outlook on future developments in the field.

Keyword(s): G protein–coupled receptor, lipidic cubic phase, serial femtosecond crystallography, structure-function, X-ray free-electron laser
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2018-05-20
2024-04-20
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Serial Femtosecond Crystallography of G Protein–Coupled Receptors
Annual Review of Biophysics 47, 377 (2018); https://doi.org/10.1146/annurev-biophys-070317-033239
/content/journals/10.1146/annurev-biophys-070317-033239
/content/journals/10.1146/annurev-biophys-070317-033239
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