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Annual Review of Physical Chemistry

Volume 65, 2014

Ion Mobility Analysis of Molecular Dynamics

Abstract

The combination of mass spectrometry and ion mobility spectrometry (IMS) employing a temperature-variable drift cell or a drift tube divided into sections to make IMS-IMS experiments possible allows information to be obtained about the molecular dynamics of polyatomic ions in the absence of a solvent. The experiments allow the investigation of structural changes of both activated and native ion populations on a timescale of 1–100 ms. Five different systems representing small and large, polar and nonpolar molecules, as well as noncovalent assemblies, are discussed in detail: a dinucleotide, a sodiated polyethylene glycol chain, the peptide bradykinin, the protein ubiquitin, and two types of peptide oligomers. Barriers to conformational interconversion can be obtained in favorable cases. In other cases, solution-like native structures can be observed, but care must be taken in the experimental protocols. The power of theoretical modeling is demonstrated.

Keyword(s): biomolecule, conformation, gas-phase structure, IMS-IMS, solution structure
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2014-04-01
2024-04-24
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/content/journals/10.1146/annurev-physchem-040513-103644
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Ion Mobility Analysis of Molecular Dynamics
Annual Review of Physical Chemistry 65, 175 (2014); https://doi.org/10.1146/annurev-physchem-040513-103644
/content/journals/10.1146/annurev-physchem-040513-103644
/content/journals/10.1146/annurev-physchem-040513-103644
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