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The unicellular green alga Chlamydomonas offers a simple life cycle, easy isolation of mutants, and a growing array of tools and techniques for molecular genetic studies. Among the principal areas of current investigation using this model system are flagellar structure and function, genetics of basal bodies (centrioles), chloroplast biogenesis, photosynthesis, light perception, cell-cell recognition, and cell cycle control. A genome project has begun with compilation of expressed sequence tag data and gene expression studies and will lead to a complete genome sequence. Resources available to the research community include wild-type and mutant strains, plasmid constructs for transformation studies, and a comprehensive on-line database.
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Sources of Strains and Information
The Chlamydomonas Genetics Center at Duke University provides cultures of wild-type and mutant strains of C. reinhardtii, C. eugametos, and C. moewusii. The web site is at http://www.biology.duke.edu/chlamy; mailing address c/o Elizabeth H. Harris, DCMB Box 91000, Duke University, Durham, NC 27708-1000. Wild-type strains of other genera are available from several major algal collections worldwide, including Culture Centre of Algae and Protozoa (CCAP; Freshwater Biology Association, The Ferry House, Ambleside, Cumbria, LA22 0LP, UK; http://wiua.nwi.ac.uk/ccap/ccaphome.html); Institute of Applied Microbiology, Tokyo (IAM; The University of Tokyo, 1-1-1 Yayoi, Bunkyou-ku, Tokyo 113, JAPAN); Sammlung von Algenkulturen (SAG; Pflanzen physiologisches Institut, Universität Göttingen, Nikolausberger Weg 18, D-3400 Göttingen, Germany (http://www.gwdg.de/botanik/phykologia/epsag.html); University of Texas Algal Collection (UTEX; Department of Botany, Austin, TX 78713-7640, Section of Molecular Cell and Developmental Biology, University of Texas, Austin, TX 78713 (http://www.bio.utexas.edu/research/utex/); University of Toronto Culture Collection (UTCC; Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada (http://www.botany.utoronto.ca/utcc/). ChlamyDB is a comprehensive database for information on Chlamydomonas and other algae in the Volvocales, including publications, sequence citations, gene descriptions, and genetic maps. This is available in a web presentation through the USDAARS Center for Bioinformatics and Comparative Genomics at Cornell University (http://arsgenome.cornell.edu/cgi-bin/WebAce/webace?db=chlamydb). Recommendations for nomenclature of Chlamydomonas genetic loci have been published by Dutcher (47) in the Trends in Genetics Genetic Nomenclature Guide. The Chlamydomonas Genetics Center coordinates availability of names for loci and mutant alleles; contact Elizabeth Harris ([email protected]) for assistance. The bionet.chlamydomonas newsgroup (http://www.bio.net:80/hypermail/CHLAMYDOMONAS/) provides a moderated forum for discussion of Chlamydomonas and other algae. The 10th International Conference on the Cell and Molecular Biology of Chlamydomonas will be held at the University of British Columbia (UBC) Conference Center in Vancouver, BC, Canada, June 11-16, 2002.