Cytoplasmic RNA Granules and Viral Infection: Video 1

A video from the 2014 review by Wei-Chih Tsai and Richard E. Lloyd, "Cytoplasmic RNA Granules and Viral Infection," from the Annual Review of Virology.

Shown: Virus-induced stress granules (small bright green foci) forming in cells infected with coxsackievirus B3 that produces dsRed fluorescent protein as a marker of robust replication. HEK cells are expressing Tia1-GFP (green) as a marker for stress granules, which translocates from the nucleus to cytoplasm as infection initiates. Expression of Tia1-GFP makes the cells more prone to stress granule formation, which generally delays or inhibits coxsackievirus B3 replication. In other infected cells, stress granules shrink or disperse after they appear, or they persist as residual pseudo–stress granules that no longer contain stalled translation complexes.