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Annual Review of Biochemistry

Volume 91, 2022

Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy

Abstract

Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for visualizing biological objects, which has been driven by advances across the entire pipeline. Developments in both single-particle analysis and in situ tomography have enabled more structures to be imaged and determined to better resolutions, at faster speeds, and with more scientists having improved access. This review highlights recent advances at each stageof the cryo-EM pipeline and provides examples of how these techniques have been used to investigate real-world problems, including antibody development against the SARS-CoV-2 spike during the recent COVID-19 pandemic.

Keyword(s): automation, cryo-EM, democratization, in situ tomography, machine learning, SARS-CoV-2
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/content/journals/10.1146/annurev-biochem-032620-110705
2022-06-21
2024-04-26
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Better, Faster, Cheaper: Recent Advances in Cryo–Electron Microscopy
Annual Review of Biochemistry 91, 1 (2022); https://doi.org/10.1146/annurev-biochem-032620-110705
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