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Abstract

Nucleic acid testing is the cornerstone of modern molecular diagnostics. This review describes the current status and future directions of molecular diagnostics, focusing on four major techniques: polymerase chain reaction (PCR), next-generation sequencing (NGS), isothermal amplification methods such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods. We explore the advantages and limitations of each technique, describe how each overlaps with or complements other techniques, and examine current clinical offerings. This review provides a broad perspective into the landscape of molecular diagnostics and highlights potential future directions in this rapidly evolving field.

Expected final online publication date for the , Volume 17 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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/content/journals/10.1146/annurev-anchem-061622-015112
2024-02-15
2024-04-23
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/content/journals/10.1146/annurev-anchem-061622-015112
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  • Article Type: Review Article
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