Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Mehmet Fatih Yanik; Christopher B. Rohde; Carlos Pardo-Martin

doi:10.1146/annurev-bioeng-071910-124703

Annual Review of Biomedical Engineering

Volume 13, 2011

Review Article

Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Mehmet Fatih Yanik^1,2, Christopher B. Rohde¹, and Carlos Pardo-Martin^3,4
View Affiliations Hide Affiliations

Affiliations: ¹Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; ²Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; ³Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139; email: [email protected] ⁴School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts 02138
Vol. 13:185-217 (Volume publication date August 2011) https://doi.org/10.1146/annurev-bioeng-071910-124703
© Annual Reviews

Abstract

Small multicellular model organisms such as the invertebrate nematode C. elegans and the vertebrate zebrafish provide unique opportunities for both basic science and pharmaceutical discovery. In recent years, there have been significant breakthroughs in technologies to manipulate and image these organisms for a variety of purposes ranging from behavioral studies of neuronal circuits to high-throughput screening. Here, we review these advancements with a particular focus on the optically transparent model organisms C. elegans and zebrafish.

Keyword(s): Caenorhabditis elegans, Danio rerio, high-throughput screening, microfluidics, zebrafish

Article metrics loading...

/content/journals/10.1146/annurev-bioeng-071910-124703

2011-08-15

2024-10-14

Full text loading...

/content/journals/10.1146/annurev-bioeng-071910-124703

Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Annual Review of Biomedical Engineering 13, 185 (2011); https://doi.org/10.1146/annurev-bioeng-071910-124703

/content/journals/10.1146/annurev-bioeng-071910-124703

Data & Media loading...

Supplementary Data

Supplemental Figure 1. Automated zebrafish injection through microforce feedback control (127–129). The embryo is positioned in an agar mold, and the micropipette is advanced to its interior. Through measurement of the microforce needed to advance the syringe, the penetration of the chorion and the cellular membrane can be sensed and the depth of injection can be controlled.

References

127. Lu Z, Chen PCY, Nam J, Ge R, Lin W. 2007. A micromanipulation system with dynamic force-feedback for automatic batch microinjection. J. Micromech. Microeng. 17(2):314–21

128. Wang W, Liu X, Gelinas D, Ciruna B, Sun Y. 2007. A fully automated robotic system for microinjection of zebrafish embryos. PLoS ONE 2(9):e862

129. Huang HB, Sun D, Mills JK, Cheng SH. 2009. Robotic cell injection system with position and force control: toward automatic batch biomanipulation. IEEE Trans. Robot. 25(3):727–37

Article Type: Review Article

Most Cited Most Cited RSS feed

- The Effect of Nanoparticle Size, Shape, and Surface Chemistry on Biological Systems
  
  Alexandre Albanese, Peter S. Tang, and Warren C.W. Chan
  
  Vol. 14 (2012), pp. 1–16
- Deep Learning in Medical Image Analysis
  
  Dinggang Shen, Guorong Wu, and Heung-Il Suk
  
  Vol. 19 (2017), pp. 221–248
- Soft Lithography in Biology and Biochemistry
  
  George M. Whitesides, Emanuele Ostuni, Shuichi Takayama, Xingyu Jiang, and Donald E. Ingber
  
  Vol. 3 (2001), pp. 335–373
- Neural Stimulation and Recording Electrodes
  
  Stuart F. Cogan
  
  Vol. 10 (2008), pp. 275–309
- Current Methods in Medical Image Segmentation¹
  
  Dzung L. Pham, Chenyang Xu, and Jerry L. Prince
  
  Vol. 2 (2000), pp. 315–337
- Microrobots for Minimally Invasive Medicine
  
  Bradley J. Nelson, Ioannis K. Kaliakatsos, and Jake J. Abbott
  
  Vol. 12 (2010), pp. 55–85
- Physics and Applications of Microfluidics in Biology
  
  David J. Beebe, Glennys A. Mensing, and Glenn M. Walker
  
  Vol. 4 (2002), pp. 261–286
- Biomaterials: Where We Have Been and Where We Are Going
  
  Buddy D. Ratner, and Stephanie J. Bryant
  
  Vol. 6 (2004), pp. 41–75
- Quantum Dots as Cellular Probes
  
  A. Paul Alivisatos, Weiwei Gu, and Carolyn Larabell
  
  Vol. 7 (2005), pp. 55–76
- Neural Tissue Engineering: Strategies for Repair and Regeneration
  
  Christine E. Schmidt, and Jennie Baier Leach
  
  Vol. 5 (2003), pp. 293–347
More Less

Annual Review of Biomedical Engineering

Volume 13, 2011

Review Article

Technologies for Micromanipulating, Imaging, and Phenotyping Small Invertebrates and Vertebrates

Abstract

Supplementary Data

Most Read This Month

Most Cited Most Cited RSS feed