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Abstract

Three-dimensional printing is a still-emerging technology with high impact for the medical community, particularly in the development of tissues for the clinic. Many types of printers are under development, including extrusion, droplet, melt, and light-curing technologies. Herein we discuss the various types of 3D printers and their strengths and weaknesses concerning tissue engineering. Despite the advantages of 3D printing, challenges remain in the development of large, clinically relevant tissues. Advancements in bioink development, printer technology, tissue vascularization, and cellular sourcing/expansion are discussed, alongside future opportunities for the field. Trends regarding in situ printing, personalized medicine, and whole organ development are highlighted.

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2022-06-07
2024-12-14
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Literature Cited

  1. 1.
    Eltom A, Zhong G, Muhammad A. 2019. Scaffold techniques and designs in tissue engineering functions and purposes: a review. Adv. Mater. Sci. Eng. 2019:3429527
    [Google Scholar]
  2. 2.
    Solchaga LA, Tognana E, Penick K, Baskaran H, Goldberg VM et al. 2006. A rapid seeding technique for the assembly of large cell/scaffold composite constructs. Tissue Eng. 12:1851–63
    [Google Scholar]
  3. 3.
    Silva MM, Cyster LA, Barry JJ, Yang XB, Oreffo RO et al. 2006. The effect of anisotropic architecture on cell and tissue infiltration into tissue engineering scaffolds. Biomaterials 27:5909–17
    [Google Scholar]
  4. 4.
    Leong MF, Rasheed MZ, Lim TC, Chian KS. 2009. In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly(D,L-lactide) scaffold fabricated by cryogenic electrospinning technique. J. Biomed. Mater. Res. A 91:231–40
    [Google Scholar]
  5. 5.
    Thevenot P, Nair A, Dey J, Yang J, Tang L 2008. Method to analyze three-dimensional cell distribution and infiltration in degradable scaffolds. Tissue Eng. C Methods 14:319–31
    [Google Scholar]
  6. 6.
    Kadota Y, Yagi H, Inomata K, Matsubara K, Hibi T et al. 2014. Mesenchymal stem cells support hepatocyte function in engineering liver grafts. Organogenesis 10:268–77
    [Google Scholar]
  7. 7.
    Scarritt ME, Pashos NC, Bunnell BA. 2015. A review of cellularization strategies for tissue engineering of whole organs. Front. Bioeng. Biotechnol. 3:43
    [Google Scholar]
  8. 8.
    Gu Z, Fu J, Lin H, He Y. 2020. Development of 3D bioprinting: from printing methods to biomedical applications. Asian J. Pharm. Sci. 15:529–57
    [Google Scholar]
  9. 9.
    Wijshoff H. 2010. The dynamics of the piezo inkjet printhead operation. Phys. Rep. 491:77–177
    [Google Scholar]
  10. 10.
    Li X, Liu B, Pei B, Chen J, Zhou D et al. 2020. Inkjet bioprinting of biomaterials. Chem. Rev. 120:10793–833
    [Google Scholar]
  11. 11.
    Li C, Faulkner-Jones A, Dun AR, Jin J, Chen P et al. 2015. Rapid formation of a supramolecular polypeptide-DNA hydrogel for in situ three-dimensional multilayer bioprinting. Angew. Chem. Int. Ed. 54:3957–61
    [Google Scholar]
  12. 12.
    Pedde RD, Mirani B, Navaei A, Styan T, Wong S et al. 2017. Emerging biofabrication strategies for engineering complex tissue constructs. Adv. Mater. 29:1606061
    [Google Scholar]
  13. 13.
    Lee VK, Dias A, Ozturk MS, Chen K, Tricomi B et al. 2015. 3D bioprinting and 3D imaging for stem cell engineering. Bioprinting in Regenerative Medicine K Turksen 33–66 Cham, Switz: Springer Int. Publ.
    [Google Scholar]
  14. 14.
    Sears NA, Seshadri DR, Dhavalikar PS, Cosgriff-Hernandez E. 2016. A review of three-dimensional printing in tissue engineering. Tissue Eng. B Rev. 22:298–310
    [Google Scholar]
  15. 15.
    Billiet T, Gevaert E, De Schryver T, Cornelissen M, Dubruel P. 2014. The 3D printing of gelatin methacylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 35:49–62
    [Google Scholar]
  16. 16.
    Chang R, Nam J, Sun W 2008. Effects of dispensing pressure and nozzle diameter on cell survival from solid freeform fabrication-based direct cell writing. Tissue Eng. A 14:41–48
    [Google Scholar]
  17. 17.
    Khalil S, Nam J, Sun W 2005. Multi-nozzle deposition for construction of 3D biopolymer tissue scaffolds. Rapid Prototyp. J. 11:9–17
    [Google Scholar]
  18. 18.
    Reid JA, Mollica PA, Johnson GD, Ogle RC, Bruno RD, Sachs PC. 2016. Accessible bioprinting: adaptation of a low-cost 3D-printer for precise cell placement and stem cell differentiation. Biofabrication 8:025017
    [Google Scholar]
  19. 19.
    Antoshin AA, Churbanov SN, Minaev NV, Zhang D, Zhang Y et al. 2019. LIFT-bioprinting, is it worth it?. Bioprinting 15:e00052
    [Google Scholar]
  20. 20.
    Willson K, Atala A, Yoo JJ. 2021. Bioprinting au natural: the biologics of bioinks. Biomolecules 11:111593
    [Google Scholar]
  21. 21.
    Mu Q, Wang L, Dunn CK, Kuang X, Duan F et al. 2017. Digital light processing 3D printing of conductive complex structures. Addit. Manuf. 18:74–83
    [Google Scholar]
  22. 22.
    Melchels FPW, Feijen J, Grijpma DW. 2010. A review on stereolithography and its applications in biomedical engineering. Biomaterials 31:6121–30
    [Google Scholar]
  23. 23.
    Fina F, Goyanes A, Gaisford S, Basit AW. 2017. Selective laser sintering (SLS) 3D printing of medicines. Int. J. Pharm. 529:285–93
    [Google Scholar]
  24. 24.
    Liu F-H, Lee R-T, Lin W-H, Liao Y-S. 2013. Selective laser sintering of bio-metal scaffold. Proc. CIRP 5:83–87
    [Google Scholar]
  25. 25.
    Buj-Corral I, Tejo-Otero A, Fenollosa-Artés F. 2020. Development of AM technologies for metals in the sector of medical implants. Metals 10:686
    [Google Scholar]
  26. [Google Scholar]
  27. 27.
    Gottlieb S. 2017. Statement by FDA Commissioner Scott Gottlieb, M.D., on FDA ushering in new era of 3D printing of medical products; provides guidance to manufacturers of medical devices. Statement, Food Drug Adm Washington, DC:
    [Google Scholar]
  28. 28.
    Faulkner-Jones A, Fyfe C, Cornelissen DJ, Gardner J, King J et al. 2015. Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D. Biofabrication 7:044102
    [Google Scholar]
  29. 29.
    Lepowsky E, Muradoglu M, Tasoglu S. 2018. Towards preserving post-printing cell viability and improving the resolution: past, present, and future of 3D bioprinting theory. Bioprinting 11:e00034
    [Google Scholar]
  30. 30.
    Nair K, Gandhi M, Khalil S, Yan KC, Marcolongo M et al. 2009. Characterization of cell viability during bioprinting processes. Biotechnol. J. 4:1168–77
    [Google Scholar]
  31. 31.
    Li M, Tian X, Zhu N, Schreyer DJ, Chen X 2010. Modeling process-induced cell damage in the biodispensing process. Tissue Eng. C 16:533–42
    [Google Scholar]
  32. 32.
    Pardo L, Wilson WC, Boland T. 2003. Characterization of patterned self-assembled monolayers and protein arrays generated by the ink-jet method. Langmuir 19:1462–66
    [Google Scholar]
  33. 33.
    Roth EA, Xu T, Das M, Gregory C, Hickman JJ, Boland T. 2004. Inkjet printing for high-throughput cell patterning. Biomaterials 25:3707–15
    [Google Scholar]
  34. 34.
    Thayer P, Martinez H, Gatenholm E. 2020. History and trends of 3D bioprinting. 3D Bioprinting: Principles and Protocols JM Crook 3–18 New York: Springer US
    [Google Scholar]
  35. 35.
    Kengla C, Atala A, Lee SJ 2015. Bioprinting of organoids. Essentials of 3D Biofabrication and Translation A Atala, JJ Yoo 271–82 Boston: Academic
    [Google Scholar]
  36. 36.
    Lawlor KT, Vanslambrouck JM, Higgins JW, Chambon A, Bishard K et al. 2021. Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation. Nat. Mater. 20:260–71
    [Google Scholar]
  37. 37.
    Yang H, Sun L, Pang Y, Hu D, Xu H et al. 2021. Three-dimensional bioprinted hepatorganoids prolong survival of mice with liver failure. Gut 70:567–74
    [Google Scholar]
  38. 38.
    Maloney E, Clark C, Sivakumar H, Yoo K, Aleman J et al. 2020. Immersion bioprinting of tumor organoids in multi-well plates for increasing chemotherapy screening throughput. Micromachines 11:208
    [Google Scholar]
  39. 39.
    Skardal A, Shupe T, Atala A. 2016. Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling. Drug Discov. Today 21:1399–411
    [Google Scholar]
  40. 40.
    Skardal A, Murphy SV, Devarasetty M, Mead I, Kang H-W et al. 2017. Multi-tissue interactions in an integrated three-tissue organ-on-a-chip platform. Sci. Rep. 7:8837
    [Google Scholar]
  41. 41.
    Achberger K, Probst C, Haderspeck J, Bolz S, Rogal J et al. 2019. Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform. eLife 8:e46188
    [Google Scholar]
  42. 42.
    Miranda CC, Fernandes TG, Diogo MM, Cabral JMS. 2018. Towards multi-organoid systems for drug screening applications. Bioengineering 5:49
    [Google Scholar]
  43. 43.
    Machino R, Matsumoto K, Taura Y, Yamasaki N, Tagagi K et al. 2015. Scaffold-free trachea tissue engineering using bioprinting. Am. J. Respir. Crit. Care Med. 191:A5343
    [Google Scholar]
  44. 44.
    Han J, Kim DS, Jang H, Kim HR, Kang HW. 2019. Bioprinting of three-dimensional dentin-pulp complex with local differentiation of human dental pulp stem cells. J. Tissue Eng. 10:2041731419845849
    [Google Scholar]
  45. 45.
    Möller T, Amoroso M, Hägg D, Brantsing C, Rotter N et al. 2017. In vivo chondrogenesis in 3D bioprinted human cell-laden hydrogel constructs. Plastic Reconstr. Surg. Glob. Open 5:e1227
    [Google Scholar]
  46. 46.
    Mayer HK, Fiechter G. 2013. Electrophoretic techniques. Compr. Anal. Chem. 60:251–78
    [Google Scholar]
  47. 47.
    Tariverdian T, Navaei T, Milan PB, Samadikuchaksaraei A, Mozafari M 2019. Functionalized polymers for tissue engineering and regenerative medicines. Advanced Functional Polymers for Biomedical Applications M Mozafari, NP Singh Chauhan 323–57 Amsterdam: Elsevier
    [Google Scholar]
  48. 48.
    Fedorovich NE, De Wijn JR, Verbout AJ, Alblas J, Dhert WJ. 2008. Three-dimensional fiber deposition of cell-laden, viable, patterned constructs for bone tissue printing. Tissue Eng. A 14:127–33
    [Google Scholar]
  49. 49.
    Livoti CM, Morgan JR. 2010. Self-assembly and tissue fusion of toroid-shaped minimal building units. Tissue Eng. A 16:2051–61
    [Google Scholar]
  50. 50.
    Ahlfeld T, Cidonio G, Kilian D, Duin S, Akkineni AR et al. 2017. Development of a clay based bioink for 3D cell printing for skeletal applications. Biofabrication 9:034103
    [Google Scholar]
  51. 51.
    Liu J, Chi J, Wang K, Liu X, Gu F 2016. Full-thickness wound healing using 3D bioprinted gelatin-alginate scaffolds in mice: a histopathological study. Int. J. Clin. Exp. Pathol. 9:11197–205
    [Google Scholar]
  52. 52.
    Shi L, Xiong L, Hu Y, Li W, Chen Z et al. 2018. Three-dimensional printing alginate/gelatin scaffolds as dermal substitutes for skin tissue engineering. Polym. Eng. Sci. 58:1782–90
    [Google Scholar]
  53. 53.
    Liu P, Shen H, Zhi Y, Si J, Shi J et al. 2019. 3D bioprinting and in vitro study of bilayered membranous construct with human cells-laden alginate/gelatin composite hydrogels. Colloids Surf. B Biointerfaces 181:1026–34
    [Google Scholar]
  54. 54.
    Dutta SD, Hexiu J, Patel DK, Ganguly K, Lim KT. 2021. 3D-printed bioactive and biodegradable hydrogel scaffolds of alginate/gelatin/cellulose nanocrystals for tissue engineering. Int. J. Biol. Macromol. 167:644–58
    [Google Scholar]
  55. 55.
    Forget A, Blaeser A, Miessmer F, Köpf M, Campos DFD et al. 2017. Mechanically tunable bioink for 3D bioprinting of human cells. Adv. Healthc. Mater. 6:1700255
    [Google Scholar]
  56. 56.
    Arya N, Forget A, Sarem M, Shastri VP. 2019. RGDSP functionalized carboxylated agarose as extrudable carriers for chondrocyte delivery. Mater. Sci. Eng. C 99:103–11
    [Google Scholar]
  57. 57.
    Verma D, Fortunati E 2019. Biopolymer processing and its composites: an introduction. Biomass, Biopolymer-Based Materials, and Bioenergy D Verma, E Fortunati, S Jain, X Zhang 3–23 Cambridge, UK: Woodhead Publ.
    [Google Scholar]
  58. 58.
    Hospodiuk M, Dey M, Sosnoski D, Ozbolat IT. 2017. The bioink: a comprehensive review on bioprintable materials. Biotechnol. Adv. 35:217–39
    [Google Scholar]
  59. 59.
    Zhang L, Hu J, Athanasiou KA. 2009. The role of tissue engineering in articular cartilage repair and regeneration. Crit. Rev. Biomed. Eng. 37:1–57
    [Google Scholar]
  60. 60.
    Yue B. 2014. Biology of the extracellular matrix: an overview. J. Glaucoma 23:S20–S23
    [Google Scholar]
  61. 61.
    Skardal A, Mack D, Kapetanovic E, Atala A, Jackson JD et al. 2012. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl. Med. 1:792–802
    [Google Scholar]
  62. 62.
    Oliveira JM. 2020. Current and future trends of silk fibroin-based bioinks in 3D printing. J. 3D Print. Med. 4:69–73
    [Google Scholar]
  63. 63.
    Gupta S, Alrabaiah H, Christophe M, Rahimi-Gorji M, Nadeem S, Bit A 2021. Evaluation of silk-based bioink during pre and post 3D bioprinting: a review. J. Biomed. Mater. Res. B 109:279–93
    [Google Scholar]
  64. 64.
    Ding H, Illsley NP, Chang RC. 2019. 3D bioprinted GelMA based models for the study of trophoblast cell invasion. Sci. Rep. 9:18854
    [Google Scholar]
  65. 65.
    Zhou X, Zhu W, Nowicki M, Miao S, Cui H et al. 2016. 3D bioprinting a cell-laden bone matrix for breast cancer metastasis study. ACS Appl. Mater. Interfaces 8:30017–26
    [Google Scholar]
  66. 66.
    Jia W, Gungor-Ozkerim PS, Zhang YS, Yue K, Zhu K et al. 2016. Direct 3D bioprinting of perfusable vascular constructs using a blend bioink. Biomaterials 106:58–68
    [Google Scholar]
  67. 67.
    Kilic Bektas C, Hasirci V 2019. Cell loaded 3D bioprinted GelMA hydrogels for corneal stroma engineering. Biomater. Sci. 8:438–49
    [Google Scholar]
  68. 68.
    Shi Y, Xing TL, Zhang HB, Yin RX, Yang SM et al. 2018. Tyrosine-doped bioink for 3D bioprinting of living skin constructs. Biomed. Mater. 13:035008
    [Google Scholar]
  69. 69.
    Ruiz-Cantu L, Gleadall A, Faris C, Segal J, Shakesheff K, Yang J 2020. Multi-material 3D bioprinting of porous constructs for cartilage regeneration. Mater. Sci. Eng. C 109:110578
    [Google Scholar]
  70. 70.
    Mouser VH, Melchels FP, Visser J, Dhert WJ, Gawlitta D, Malda J. 2016. Yield stress determines bioprintability of hydrogels based on gelatin-methacryloyl and gellan gum for cartilage bioprinting. Biofabrication 8:035003
    [Google Scholar]
  71. 71.
    Bejleri D, Streeter BW, Nachlas ALY, Brown ME, Gaetani R et al. 2018. A bioprinted cardiac patch composed of cardiac-specific extracellular matrix and progenitor cells for heart repair. Adv. Healthc. Mater. 7:e1800672
    [Google Scholar]
  72. 72.
    Koti P, Muselimyan N, Mirdamadi E, Asfour H, Sarvazyan NA. 2019. Use of GelMA for 3D printing of cardiac myocytes and fibroblasts. J. 3D Print. Med. 3:11–22
    [Google Scholar]
  73. 73.
    Bhise NS, Manoharan V, Massa S, Tamayol A, Ghaderi M et al. 2016. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication 8:014101
    [Google Scholar]
  74. 74.
    Ma X, Qu X, Zhu W, Li Y-S, Yuan S et al. 2016. Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting. PNAS 113:2206–11
    [Google Scholar]
  75. 75.
    Cuvellier M, Ezan F, Oliveira H, Rose S, Fricain JC et al. 2021. 3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model. Biomaterials 269:120611
    [Google Scholar]
  76. 76.
    Gungor-Ozkerim PS, Inci I, Zhang YS, Khademhosseini A, Dokmeci MR 2018. Bioinks for 3D bioprinting: an overview. Biomater. Sci. 6:915–46
    [Google Scholar]
  77. 77.
    Costa RM, Rauf S, Hauser CAE. 2017. Towards biologically relevant synthetic designer matrices in 3D bioprinting for tissue engineering and regenerative medicine. Curr. Opin. Biomed. Eng. 2:90–98
    [Google Scholar]
  78. 78.
    Parak A, Pradeep P, du Toit LC, Kumar P, Choonara YE, Pillay V. 2019. Functionalizing bioinks for 3D bioprinting applications. Drug Discov. Today 24:198–205
    [Google Scholar]
  79. 79.
    Jorgensen AM, Varkey M, Gorkun A, Clouse C, Xu L et al. 2020. Bioprinted skin recapitulates normal collagen remodeling in full-thickness wounds. Tissue Eng. A 26:512–26
    [Google Scholar]
  80. 80.
    Gao G, Lee JH, Jang J, Lee DH, Kong JS et al. 2017. Tissue engineered bio-blood-vessels constructed using a tissue-specific bioink and 3D coaxial cell printing technique: a novel therapy for ischemic disease. Adv. Funct. Mater. 27:1700798
    [Google Scholar]
  81. 81.
    Yu C, Ma X, Zhu W, Wang P, Miller KL et al. 2019. Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix. Biomaterials 194:1–13
    [Google Scholar]
  82. 82.
    Kaully T, Kaufman-Francis K, Lesman A, Levenberg S. 2009. Vascularization—the conduit to viable engineered tissues. Tissue Eng. B Rev. 15:159–69
    [Google Scholar]
  83. 83.
    Bertassoni LE, Cecconi M, Manoharan V, Nikkhah M, Hjortnaes J et al. 2014. Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab Chip 14:2202–11
    [Google Scholar]
  84. 84.
    Nashimoto Y, Hayashi T, Kunita I, Nakamasu A, Torisawa Y et al. 2017. Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. Integr. Biol. 9:506–18
    [Google Scholar]
  85. 85.
    Moya M, Tran D, George SC 2013. An integrated in vitro model of perfused tumor and cardiac tissue. Stem Cell Res. Ther. 4:S15
    [Google Scholar]
  86. 86.
    Ju YM, Choi JS, Atala A, Yoo JJ, Lee SJ. 2010. Bilayered scaffold for engineering cellularized blood vessels. Biomaterials 31:4313–21
    [Google Scholar]
  87. 87.
    Assmann A, Delfs C, Munakata H, Schiffer F, Horstkötter K et al. 2013. Acceleration of autologous in vivo recellularization of decellularized aortic conduits by fibronectin surface coating. Biomaterials 34:6015–26
    [Google Scholar]
  88. 88.
    Dall'Olmo L, Zanusso I, Di Liddo R, Chioato T, Bertalot T et al. 2014. Blood vessel–derived acellular matrix for vascular graft application. Biomed. Res. Int. 2014:685426
    [Google Scholar]
  89. 89.
    Jung Y, Ji H, Chen Z, Fai Chan H, Atchison L et al. 2015. Scaffold-free, human mesenchymal stem cell–based tissue engineered blood vessels. Sci. Rep. 5:15116
    [Google Scholar]
  90. 90.
    L'Heureux N, Pâquet S, Labbé R, Germain L, Auger FA. 1998. A completely biological tissue-engineered human blood vessel. FASEB J. 12:47–56
    [Google Scholar]
  91. 91.
    Bos GW, Poot AA, Beugeling T, van Aken WG, Feijen J. 1998. Small-diameter vascular graft prostheses: current status. Arch. Physiol. Biochem. 106:15
    [Google Scholar]
  92. 92.
    Seifu DG, Purnama A, Mequanint K, Mantovani D. 2013. Small-diameter vascular tissue engineering. Nat. Rev. Cardiol. 10:410–21
    [Google Scholar]
  93. 93.
    McAllister TN, Maruszewski M, Garrido SA, Wystrychowski W, Dusserre N et al. 2009. Effectiveness of haemodialysis access with an autologous tissue-engineered vascular graft: a multicentre cohort study. Lancet 373:1440–46
    [Google Scholar]
  94. 94.
    Grigoryan B, Paulsen SJ, Corbett DC, Sazer DW, Fortin CL et al. 2019. Multivascular networks and functional intravascular topologies within biocompatible hydrogels. Science 364:458–64
    [Google Scholar]
  95. 95.
    Zhu W, Qu X, Zhu J, Ma X, Patel S et al. 2017. Direct 3D bioprinting of prevascularized tissue constructs with complex microarchitecture. Biomaterials 124:106–15
    [Google Scholar]
  96. 96.
    Ye W, Li H, Yu K, Xie C, Wang P et al. 2020. 3D printing of gelatin methacrylate-based nerve guidance conduits with multiple channels. Mater. Des. 192:108757
    [Google Scholar]
  97. 97.
    Yang Y, Zhou Y, Lin X, Yang Q, Yang G 2020. Printability of external and internal structures based on digital light processing 3D printing technique. Pharmaceutics 12:207
    [Google Scholar]
  98. 98.
    Wang Y, Wang Y, Mei D. 2020. Scalable printing of bionic multiscale channel networks through digital light processing-based three-dimensional printing process. 3D Print. . Addit. Manuf. 7:115–25
    [Google Scholar]
  99. 99.
    O'Bryan C, Bhattacharjee T, Niemi S, Balachandar S, Baldwin N et al. 2017. Three-dimensional printing with sacrificial materials for soft matter manufacturing. MRS Bull 42:8571–77
    [Google Scholar]
  100. 100.
    Miller JS, Stevens KR, Yang MT, Baker BM, Nguyen D-HT et al. 2012. Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues. Nat. Mater. 11:768–74
    [Google Scholar]
  101. 101.
    Liu J, Li Y, Fan H, Zhu Z, Jiang J et al. 2010. Iron oxide-based nanotube arrays derived from sacrificial template-accelerated hydrolysis: large-area design and reversible lithium storage. Chem. Mater. 22:212–17
    [Google Scholar]
  102. 102.
    Kolesky DB, Truby RL, Gladman AS, Busbee TA, Homan KA, Lewis JA. 2014. 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs. Adv. Mater. 26:3124–30
    [Google Scholar]
  103. 103.
    Zhang Y, Yu Y, Chen H, Ozbolat IT. 2013. Characterization of printable cellular micro-fluidic channels for tissue engineering. Biofabrication 5:025004
    [Google Scholar]
  104. 104.
    Christensen K, Xu C, Chai W, Zhang Z, Fu J, Huang Y. 2015. Freeform inkjet printing of cellular structures with bifuractions. Biotechnol. Bioeng. 112:1047–55
    [Google Scholar]
  105. 105.
    Hinton TJ, Jallerat Q, Palchesko RN, Park JH, Grodzicki MS et al. 2015. Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels. Sci. Adv. 1: https://doi.org/10.1126/sciadv.1500758
    [Crossref] [Google Scholar]
  106. 106.
    Yifan Z, Tori S, Senthilkuman D, Morley C, Taylor C, Angelini T. 2021. 3D printed collagen structures at low concentrations supported by jammed microgels. Bioprinting 21:e00121
    [Google Scholar]
  107. 107.
    Mirdamadi E, Tashman J, Shiwarski D, Palchesko R, Feinberg A. 2020. Fresh 3D bioprinting a full-size model of the human heart. ACS Biomater. Sci. Eng. 6:116453–59
    [Google Scholar]
  108. 108.
    Simaria AS, Hassan S, Varadaraju H, Rowley J, Warren K et al. 2014. Allogeneic cell therapy bioprocess economics and optimization: single-use cell expansion technologies. Biotechnol. Bioeng. 111:69–83
    [Google Scholar]
  109. 109.
    Geraghty EM, Boone JM, McGahan JP, Jain K. 2004. Normal organ volume assessment from abdominal CT. Abdom. Imaging 29:482–90
    [Google Scholar]
  110. 110.
    Blüml G 2007. Microcarrier cell culture technology. Animal Cell Biotechnology: Methods and Protocols R Pörtner 149–78 Totowa, NJ: Humana
    [Google Scholar]
  111. 111.
    Shi F, Wang Y-C, Zhao T-Z, Zhang S, Du T-Y et al. 2012. Effects of simulated microgravity on human umbilical vein endothelial cell angiogenesis and role of the PI3K-Akt-eNOS signal pathway. PLOS ONE 7:e40365
    [Google Scholar]
  112. 112.
    Shi F, Zhao TZ, Wang YC, Cao XS, Yang CB et al. 2016. The impact of simulated weightlessness on endothelium-dependent angiogenesis and the role of caveolae/caveolin-1. Cell. Physiol. Biochem. 38:502–13
    [Google Scholar]
  113. 113.
    Carlsson SIM, Bertilaccio MTS, Ballabio E, Maier JAM. 2003. Endothelial stress by gravitational unloading: effects on cell growth and cytoskeletal organization. Biochim. Biophys. Acta 1642:173–79
    [Google Scholar]
  114. 114.
    Coward SM, Selden C, Mantalaris A, Hodgson HJF. 2005. Proliferation rates of HepG2 cells encapsulated in alginate are increased in a microgravity environment compared with static cultures. Artif. Organs 29:152–58
    [Google Scholar]
  115. 115.
    Kaur G, Dufour JM. 2012. Cell lines: Valuable tools or useless artifacts. Spermatogenesis 2:1–5
    [Google Scholar]
  116. 116.
    Singh S, Choudhury D, Yu F, Mironov V, Naing MW. 2020. In situ bioprinting—bioprinting from benchside to beside?. Acta Biomater. 101:14–25
    [Google Scholar]
  117. 117.
    Binder KW, Zhao W, Aboushwareb T, Dice D, Atala A, Yoo JJ. 2010. In situ bioprinting of the skin for burns. J. Am. Coll. Surg. 211:S76
    [Google Scholar]
  118. 118.
    Cohen DL, Lipton JI, Bonassar LJ, Lipson H. 2010. Additive manufacturing for in situ repair of osteochondral defects. Biofabrication 2:035004
    [Google Scholar]
  119. 119.
    Duchi S, Onofrillo C, O'Connell CD, Blanchard R, Augustine C et al. 2017. Handheld co-axial bioprinting: application to in situ surgical cartilage repair. Sci. Rep. 7:5837
    [Google Scholar]
  120. 120.
    Chen Y, Zhang J, Liu X, Wang S, Tao J et al. 2020. Noninvasive in vivo 3D bioprinting. Sci. Adv. 6:eaba7406
    [Google Scholar]
  121. 121.
    Hu J, Tomov M, Buikema J, Chen C, Mahmoudi M et al. 2018. Cardiovascular tissue bioprinting: physical and chemical processes. Appl. Phys. Rev. 5:041106
    [Google Scholar]
  122. 122.
    Wang C, Tan X, Liu E, Tor S. 2018. Process parameter optimization and mechanical properties for additively manufactured stainless steel 316L parts by selective electron beam melting. Mater. Design 147:157–66
    [Google Scholar]
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