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Annual Review of Phytopathology

Volume 60, 2022

Point-of-Care DNA Amplification for Disease Diagnosis and Management

Abstract

Early detection of pests and pathogens is of paramount importance in reducing agricultural losses. One approach to early detection is point-of-care (POC) diagnostics, which can provide early warning and therefore allow fast deployment of preventive measures to slow down the establishment of crop diseases. Among the available diagnostic technologies, nucleic acid amplification–based diagnostics provide the highest sensitivity and specificity, and those technologies that forego the requirement for thermocycling show the most potential for use at POC. In this review, I discuss the progress, advantages, and disadvantages of the established and most promising POC amplification technologies. The success and usefulness of POC amplification are ultimately dependent on the availability of POC-friendly nucleic acid extraction methods and amplification readouts, which are also briefly discussed in the review.

Keyword(s): crop diagnostics, field disease diagnostics, isothermal amplification, point-of-care amplification, point-of-care diagnostics
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/content/journals/10.1146/annurev-phyto-021621-115027
2022-08-26
2025-02-18
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/content/journals/10.1146/annurev-phyto-021621-115027
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Point-of-Care DNA Amplification for Disease Diagnosis and Management
Annual Review of Phytopathology 60, 1 (2022); https://doi.org/10.1146/annurev-phyto-021621-115027
/content/journals/10.1146/annurev-phyto-021621-115027
/content/journals/10.1146/annurev-phyto-021621-115027
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