1932

Abstract

Abstract

Single-molecule spectroscopy has emerged as a valuable tool in probing kinetics and dynamic equilibria in adsorption because advances in instrumentation and technology have enabled researchers to obtain high signal-to-noise ratios for common dyes at room temperature. Single-molecule spectroscopy was applied to the study of an important problem in chromatography: peak broadening and asymmetry in the chromatograms of pharmaceuticals, peptides, and proteins. Using DiI, a cationic dye that exhibits the same problematic chromatographic behavior, investigators showed that the adsorption sites that cause chromatographic problems are located at defects on the silica crystal surface.

Loading

Article metrics loading...

/content/journals/10.1146/annurev.physchem.58.032806.104427
2007-05-05
2024-06-16
Loading full text...

Full text loading...

/content/journals/10.1146/annurev.physchem.58.032806.104427
Loading
/content/journals/10.1146/annurev.physchem.58.032806.104427
Loading

Data & Media loading...

  • Article Type: Review Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error