1932

Abstract

At birth, fetal distal lung epithelial (FDLE) cells switch from active chloride secretion to active sodium (Na+) reabsorption. Sodium ions Senter the FDLE and alveolar type II (ATII) cells mainly through apical nonselective cation and Na+-selective channels, with conductances of 4–26 pS (picoSiemens) in FDLE and 20–25 pS in ATII cells. All these channels are inhibited by amiloride with a 50% inhibitory concentration of <1 μM, and some are also inhibited by [N-ethyl-N-isopropyl]-2′-4′-amiloride (50% inhibitory concentration of <1 μM). Both FDLE and ATII cells contain the α-, β-, and γ-rENaC (rat epithelial Na+ channels) mRNAs; reconstitution of an ATII cell Na+-channel protein into lipid bilayers revealed the presence of 25-pS Na+ single channels, inhibited by amiloride and [N-ethyl-N-isopropyl]-2′-4′-amiloride. A variety of agents, including cAMP, oxygen, glucocorticoids, and in some cases Ca2+, increased the activity and/or rENaC mRNA levels. The phenotypic properties of these channels differ from those observed in other Na+-absorbing epithelia. Pharmacological blockade of alveolar Na+ transport in vivo, as well as experiments with newborn α-rENaC knock-out mice, demonstrate the importance of active Na+ transport in the reabsorption of fluid from the fetal lung and in reabsorbing alveolar fluid in the injured adult lung. Indeed, in a number of inflammatory diseases, increased production of reactive oxygen-nitrogen intermediates, such as peroxynitrite (ONOO), may damage ATII and FDLE Na+ channels, decrease Na+ reabsorption in vivo, and thus contribute to the formation of alveolar edema.

Loading

Article metrics loading...

/content/journals/10.1146/annurev.physiol.61.1.627
1999-03-01
2024-06-16
Loading full text...

Full text loading...

/content/journals/10.1146/annurev.physiol.61.1.627
Loading
/content/journals/10.1146/annurev.physiol.61.1.627
Loading

Data & Media loading...

  • Article Type: Review Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error