Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue: Video 12

A video from the 2014 review by Hai Qi, Wolfgang Kastenmüller, and Ronald N. Germain, "Spatiotemporal Basis of Innate and Adaptive Immunity in Secondary Lymphoid Tissue," from the Annual Review of Cell and Developmental Biology.

Shown: (a) Recruitment of antigen-unspecific memory CD8⁺ T cells to the SCS area

Popliteal LNs from MVA WT-infected (footpad) mice harboring memory OT-I T cells were surgically exposed and analyzed by two-photon microscopy. Video shows recruitment to the SCS and swarming of memory OT-I T cells in the absence of cognate antigen.

(b) Recruitment of antigen-unspecific memory CD8⁺ T cells to the SCS area depends on CXCR3

Popliteal LNs from CD11cYFP mice, infected with MVA WT (footpad) harboring WT and Cxcr3 ^−/− memory OT-I T cells were surgically exposed and analyzed by two-photon microscopy. Video shows differential migration patterns of WT versus Cxcr3 ^−/− memory OT-I T cells and swarming behavior of WT but not Cxcr3 ^−/− memory OT-I T cells around DCs.

Original publication: Kastenmüller W, Brandes M, Wang Z, Herz J, Egen JG, Germain RN. 2013. Peripheral prepositioning and local CXCL9 chemokine-mediated guidance orchestrate rapid memory CD8⁺ T cell responses in the lymph node. Immunity 38:502–13