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Annual Review of Analytical Chemistry

Volume 9, 2016

In Situ and In Vivo Molecular Analysis by Coherent Raman Scattering Microscopy

Abstract

Coherent Raman scattering (CRS) microscopy is a high-speed vibrational imaging platform with the ability to visualize the chemical content of a living specimen by using molecular vibrational fingerprints. We review technical advances and biological applications of CRS microscopy. The basic theory of CRS and the state-of-the-art instrumentation of a CRS microscope are presented. We further summarize and compare the algorithms that are used to separate the Raman signal from the nonresonant background, to denoise a CRS image, and to decompose a hyperspectral CRS image into concentration maps of principal components. Important applications of single-frequency and hyperspectral CRS microscopy are highlighted. Potential directions of CRS microscopy are discussed.

Keyword(s): biomedical imaging, Raman scattering, vibrational spectroscopy
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/content/journals/10.1146/annurev-anchem-071015-041627
2016-06-12
2025-04-22
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In Situ and In Vivo Molecular Analysis by Coherent Raman Scattering Microscopy
Annual Review of Analytical Chemistry 9, 69 (2016); https://doi.org/10.1146/annurev-anchem-071015-041627
/content/journals/10.1146/annurev-anchem-071015-041627
/content/journals/10.1146/annurev-anchem-071015-041627
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