1932

Abstract

My early life was challenging, and not conducive to the study of science, but my first introduction to viruses was an epiphany for me. I spent the whole of my career dedicated to understanding viruses, driven largely by curiosity. This led me down many different avenues of study, and to work with many wonderful colleagues, most of whom remain friends. Some highlights of my career include the discovery of a mutualistic three-way symbiosis involving a virus, a fungus, and a plant; genetic mapping of a pathogenicity gene in tomato; uncovering a virus in 1,000-year-old corncobs; exploring virus biodiversity in wild plants; and establishing a system to use a fungal virus to understand the epidemiology of its host.

Loading

Article metrics loading...

/content/journals/10.1146/annurev-virology-100520-013446
2022-09-29
2024-05-11
Loading full text...

Full text loading...

/deliver/fulltext/virology/9/1/annurev-virology-100520-013446.html?itemId=/content/journals/10.1146/annurev-virology-100520-013446&mimeType=html&fmt=ahah

Literature Cited

  1. 1.
    Roossinck MJ, Jameel S, Loukin SH, Siddiqui A. 1986. Expression of hepatitis B viral core region in mammalian cells. Mol. Cell. Biol. 6:1393–400
    [Google Scholar]
  2. 2.
    Roossinck MJ, Siddiqui A. 1987. In vivo phosphorylation and protein analysis of hepatitis B virus core antigen. J. Virol. 61:955–61
    [Crossref] [Google Scholar]
  3. 3.
    Viswanathan U, Mani N, Hu Z, Ban H, Du Y et al. 2020. Targeting the multifunctional HBV core protein as a potential cure for chronic hepatitis B. Antiviral Res. 182:104917
    [Crossref] [Google Scholar]
  4. 4.
    Everson GT, Polokoff MA. 1986. HepG2. A human hepatoblastoma cell line exhibiting defects in bile acid synthesis and conjugation. J. Biol. Chem. 261:2197–201
    [Crossref] [Google Scholar]
  5. 5.
    Roossinck MJ, Palukaitis P. 1990. Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA 1 of cucumber mosaic virus. Mol. Plant-Microbe Interact. 3:188–92
    [Crossref] [Google Scholar]
  6. 6.
    Palukaitis P, Roossinck MJ, Shintaku MH, Sleat DE. 1991. Mapping functional domains in cucumber mosaic virus and its satellite RNAs. Can. J. Plant Pathol. 13:155–62
    [Crossref] [Google Scholar]
  7. 7.
    Roossinck MJ. 1991. Temperature-sensitive replication of cucumber mosaic virus in muskmelon (Cucumis melo cv. Iroquois), maps to RNA 1 of a slow strain. J. Gen. Virol. 72:1747–50
    [Crossref] [Google Scholar]
  8. 8.
    Roossinck MJ, Palukaitis P. 1991. Differential replication in zucchini squash of a cucumber mosaic virus satellite RNA maps to RNA 1 of the helper virus. Virology 181:371–73
    [Crossref] [Google Scholar]
  9. 9.
    Sivakumaran K, Bao Y, Roossinck MJ, Kao CC. 2000. Recognition of the core RNA promoter for minus-strand RNA synthesis by the replicase of Brome mosaicvirus and Cucumber mosaic virus. J. Virol. 74:10323–31
    [Crossref] [Google Scholar]
  10. 10.
    Sivakumaran K, Chen M-H, Roossinck MJ, Kao CC. 2002. Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A. Mol. Plant Pathol. 3:43–52
    [Crossref] [Google Scholar]
  11. 11.
    Graves MV, Roossinck MJ. 1995. Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus. J. Virol. 69:4746–51
    [Crossref] [Google Scholar]
  12. 12.
    Rodríguez-Alvarado G, Roossinck MJ. 1997. Structural analysis of a necrogenic strain of cucumber mosaic cucumovirus satellite RNA in planta. Virology 236:155–66
    [Crossref] [Google Scholar]
  13. 13.
    Xu P, Roossinck MJ. 2000. Cucumber mosaic virus D satellite RNA-induced programmed cell death in tomato. Plant Cell 12:1079–92
    [Google Scholar]
  14. 14.
    Xu P, Blancaflor EB, Roossinck MJ. 2003. In spite of induced multiple defense responses, tomato plants infected with Cucumber mosaic virus and D satellite RNA succumb to systemic necrosis. Mol. Plant-Microbe Interact. 16:467–76
    [Crossref] [Google Scholar]
  15. 15.
    Xu P, Rogers SJ, Roossinck MJ. 2004. Expression of antiapoptotic genes bcl-xL and ced-9 in tomato enhances tolerance to virus-induced necrosis and abiotic stress. PNAS 101:15805–10
    [Crossref] [Google Scholar]
  16. 16.
    Irian S, Xu P, Dai X, Zhao PX, Roossinck MJ. 2007. Regulation of a virus-induced lethal disease in tomato revealed by LongSAGE analysis. Mol. Plant-Microbe Interact. 20:1477–88
    [Crossref] [Google Scholar]
  17. 17.
    Xu P, Wang H, Coker F, Ma J-y, Tang Y et al. 2012. Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection. Mol. Plant-Microbe Interact. 25:1034–44
    [Crossref] [Google Scholar]
  18. 18.
    Hord MJ, García A, Villalobos H, Rivera C, Macaya G, Roossinck MJ. 2001. Field survey of Cucumber mosaic virus subgroups I and II in crop plants in Costa Rica. Plant Dis 85:952–54
    [Crossref] [Google Scholar]
  19. 19.
    Dodds JA, Morris TJ, Jordan RL. 1984. Plant viral double-stranded RNA. Annu. Rev. Phytopathol. 22:151–68
    [Crossref] [Google Scholar]
  20. 20.
    Margulies M, Egholm M, Altman WE, Attiya S, Bader JS et al. 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:378–80
    [Crossref] [Google Scholar]
  21. 21.
    Wren JD, Roossinck MJ, Nelson RS, Sheets K, Palmer MW, Melcher U. 2006. Plant virus biodiversity and ecology. PLOS Biol. 4:e80
    [Crossref] [Google Scholar]
  22. 22.
    Roossinck MJ. 2011. Environmental viruses from biodiversity to ecology. Curr. Opin. Virol. 1:50–51
    [Crossref] [Google Scholar]
  23. 23.
    Roossinck MJ. 2011. The big unknown: plant virus biodiversity. Curr. Opin. Virol. 1:63–67
    [Crossref] [Google Scholar]
  24. 24.
    Roossinck MJ. 2012. Plant virus metagenomics: biodiversity and ecology. Annu. Rev. Genet. 46:357–67
    [Crossref] [Google Scholar]
  25. 25.
    Roossinck MJ, Saha P, Wiley GB, Quan J, White JD et al. 2010. Ecogenomics: using massively parallel pyrosequencing to understand virus ecology. Mol. Ecol. 19:S181–88
    [Crossref] [Google Scholar]
  26. 26.
    Min B-E, Feldman TS, Ali A, Wiley G, Muthukumar V et al. 2012. Molecular characterization, ecology, and epidemiology of a novel tymovirus in Asclepias viridis from Oklahoma. Phytopathology 102:166–76
    [Crossref] [Google Scholar]
  27. 27.
    Stobbe AH, Melcher U, Palmer MW, Roossinck MJ, Shen G. 2012. Co-divergence and host-switching in the evolution of tobamoviruses. J. Gen. Virol. 93:408–18
    [Crossref] [Google Scholar]
  28. 28.
    Stobbe AH, Roossinck MJ. 2014. Plant virus metagenomics: what we know and why we need to know more. Front. Plant Sci. 5:150
    [Crossref] [Google Scholar]
  29. 29.
    Thapa V, McGlinn DJ, Melcher U, Palmer MW, Roossinck MJ. 2015. Determinants of taxonomic composition of plant viruses at the Nature Conservancy's Tallgrass Prairie Preserve, Oklahoma. Virus Evol 1:vev007
    [Crossref] [Google Scholar]
  30. 30.
    Feldman TS, Morsy MR, Roossinck MJ. 2012. Are communities of microbial symbionts more diverse than communities of microbial hosts?. Fungal Biol 116:465–77
    [Crossref] [Google Scholar]
  31. 31.
    Roossinck MJ. 2010. Lifestyles of plant viruses. Philos. Trans. R. Soc. B 365:1899–905
    [Crossref] [Google Scholar]
  32. 32.
    Roossinck MJ 2012. Persistent plant viruses: molecular hitchhikers or epigenetic elements?. Viruses: Essential Agents of Life G Witzany 177–86 Dordrecht, Neth: Springer
    [Google Scholar]
  33. 33.
    Roossinck MJ. 2019. Evolutionary and ecological links between plant and fungal viruses. New Phytol 221:86–92
    [Crossref] [Google Scholar]
  34. 34.
    Okada R, Kiyota E, Sabanadzovic S, Moriyama H, Fukuhara T et al. 2011. Bell pepper endornavirus: molecular and biological properties, and occurrence in the genus Capsicum. J. Gen. Virol. 92:2664–73
    [Crossref] [Google Scholar]
  35. 35.
    Roossinck MJ, Sabanadzovic S, Okada R, Valverde RA. 2011. The remarkable evolutionary history of endornaviruses. J. Gen. Virol. 92:2674–78
    [Crossref] [Google Scholar]
  36. 36.
    Safari M, Roossinck MJ. 2018. Coevolution of a persistent plant virus and its pepper hosts. Mol. Plant-Microbe Interact 31:766–76
    [Crossref] [Google Scholar]
  37. 37.
    Vaudo AD, Tooker JF, Patch HM, Biddinger DJ, Coccia M et al. 2020. Pollen protein: Lipid macronutrient ratios may guide broad patterns of bee species floral preferences. Insects 11:132
    [Crossref] [Google Scholar]
  38. 38.
    Peyambari M, Habibi MK, Fotouhifar K-B, Dizakju A, Roossinck MJ. 2014. Molecular characterization of a novel putative partitivirus infecting Cytospora sacchari, a plant pathogenic fungus. Plant Pathol. J. 30:151–58
    [Crossref] [Google Scholar]
  39. 39.
    Peyambari M, Guan S, Roossinck MJ. 2021. RdRp or RT, that is the question. Mol. Biol. Evol. 38:5082–91
    [Crossref] [Google Scholar]
  40. 40.
    Peyambari M, Warner S, Stoler N, Ranier D, Roossinck MJ. 2019. A 1,000-year-old RNA virus. J. Virol. 93:e01188–18
    [Crossref] [Google Scholar]
  41. 41.
    White PS, Morales FJ, Roossinck MJ. 1995. Interspecific reassortment in the evolution of a cucumovirus. Virology 207:334–37
    [Crossref] [Google Scholar]
  42. 42.
    Roossinck MJ, Zhang L, Hellwald K-H. 1999. Rearrangements in the 5′ nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73:6752–58
    [Crossref] [Google Scholar]
  43. 43.
    Roossinck MJ. 2002. Evolutionary history of Cucumber mosaic virus deduced by phylogenetic analyses. J. Virol. 76:3382–87
    [Crossref] [Google Scholar]
  44. 44.
    Schneider WL, Roossinck MJ. 2000. Evolutionarily related Sindbis-like plant viruses maintain different levels of population diversity in a common host. J. Virol. 74:3130–34
    [Crossref] [Google Scholar]
  45. 45.
    Schneider WL, Roossinck MJ. 2001. Genetic diversity in RNA viral quasispecies is controlled by host-virus interactions. J. Virol. 75:6566–71
    [Crossref] [Google Scholar]
  46. 46.
    Pita JS, Roossinck MJ. 2013. Mapping viral functional domains for genetic diversity in plants. J. Virol. 87:790–97
    [Crossref] [Google Scholar]
  47. 47.
    Pita JS, Roossinck MJ. 2013. Fixation of emerging interviral recombinants in Cucumber mosaic virus populations. J. Virol. 87:1264–69
    [Crossref] [Google Scholar]
  48. 48.
    Pita JS, Morris V, Roossinck MJ. 2015. Mutation and recombination frequencies reveal a biological contrast within strains of Cucumber mosaic virus. J. Virol. 89:6817–23
    [Crossref] [Google Scholar]
  49. 49.
    Ouedraogo RS, Pita JS, Somda IP, Traore O, Roossinck MJ. 2019. Impact of cultivated hosts on recombination of Cucumber mosaic virus. J. Virol. 93:e01770–18
    [Crossref] [Google Scholar]
  50. 50.
    Li H, Roossinck MJ. 2004. Genetic bottlenecks reduce population variation in an experimental RNA virus population. J. Virol. 78:10582–87
    [Crossref] [Google Scholar]
  51. 51.
    Ali A, Roossinck MJ. 2010. Genetic bottlenecks during systemic movement of Cucumber mosaic virus vary in different host plants. Virology 404:279–83
    [Crossref] [Google Scholar]
  52. 52.
    Ali A, Li H, Schneider WL, Sherman DJ, Gray S et al. 2006. Analysis of genetic bottlenecks during horizontal transmission of Cucumber mosaic virus. J. Virol. 80:8345–50
    [Crossref] [Google Scholar]
  53. 53.
    Palukaitis P, Roossinck MJ. 1995. Variation in the hypervariable region of cucumber mosaic virus satellite RNAs is affected by the helper virus and the initial sequence context. Virology 206:765–68
    [Crossref] [Google Scholar]
  54. 54.
    Roossinck MJ, Palukaitis P. 1995. Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs. J. Mol. Evol. 40:25–29
    [Crossref] [Google Scholar]
  55. 55.
    Palukaitis P, Roossinck MJ. 1996. Spontaneous change of a benign satellite RNA of cucumber mosaic virus to a pathogenic variant. Nat. Biotechnol. 14:1264–68
    [Crossref] [Google Scholar]
  56. 56.
    Pita JS, deMiranda JR, Schneider WL, Roossinck MJ. 2007. Environment determines fidelity for an RNA virus replicase. J. Virol. 81:9072–77
    [Crossref] [Google Scholar]
  57. 57.
    Mochizuki T, Ohara R, Roossinck MJ. 2018. Large-scale synonymous substitutions in Cucumber mosaic virus RNA 3 facilitate amino acid mutations in the coat protein. J. Virol. 92:e01007–18
    [Crossref] [Google Scholar]
  58. 58.
    Redman RS, Sheehan KB, Stout RG, Rodriguez RJ, Henson JM. 2002. Thermotolerance generated by plant/fungal symbiosis. Science 298:1581
    [Crossref] [Google Scholar]
  59. 59.
    Márquez LM, Redman RS, Rodriguez RJ, Roossinck MJ. 2007. A virus in a fungus in a plant: three-way symbiosis required for thermal tolerance. Science 315:513–15
    [Crossref] [Google Scholar]
  60. 60.
    Morsy MR, Oswald J, He J, Tang Y, Roossinck MJ. 2010. Teasing apart a three-way symbiosis: transcriptome analyses of Curvularia protuberata in response to viral infection and heat stress. Biochem. Biophys. Res. Commun. 401:225–30
    [Crossref] [Google Scholar]
  61. 61.
    Bao X, Roossinck MJ. 2013. A life history view of mutualistic viral symbioses: quantity or quality for cooperation?. Curr. Opin. Micro. 16:514–18
    [Crossref] [Google Scholar]
  62. 62.
    Safari M, Roossinck MJ. 2014. How does the genome structure and lifestyle of a virus affect its population variation?. Curr. Opin. Virol. 9:39–44
    [Crossref] [Google Scholar]
  63. 63.
    Xu P, Chen F, Mannas JP, Feldman T, Sumner LW, Roossinck MJ. 2008. Virus infection improves drought tolerance. New Phytol. 180:911–21
    [Crossref] [Google Scholar]
  64. 64.
    Thapa V, Turner GG, Hafenstein S, Overton BE, Vanderwolf KJ, Roossinck MJ. 2016. Using a novel partitivirus in Pseudogymnoascus destructans to understand the epidemiology of white-nose syndrome. PLOS Pathog 12:e1006076
    [Crossref] [Google Scholar]
  65. 65.
    Thapa V, Turner GG, Roossinck MJ. 2021. Phylogeographic analysis of Pseudogymnoascus destructans partitivirus-pa explains the spread dynamics of white-nose syndrome in North America. PLOS Pathog. 17:3e1009236
    [Crossref] [Google Scholar]
  66. 66.
    Thapa V, Keller NP, Roossinck MJ. 2022. Evaluation of virus-free and wild-type isolates of Pseudogymnoascus destructans using a porcine ear model. mSphere. In press
    [Google Scholar]
/content/journals/10.1146/annurev-virology-100520-013446
Loading
/content/journals/10.1146/annurev-virology-100520-013446
Loading

Data & Media loading...

  • Article Type: Review Article
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error